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HIV-1 P17, Core, Strain IIIB (HIV) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635570

p17 HIV-1 IIIB (E. coli) conjugated to Colloidal Gold (25nm mean particle size). More details

$1385 / 1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIV-1 p17, Core, Strain IIIB, Recombinant Protein
Full Product Name
HIV-1 p17, Core, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Gold)
Host
E Coli
Form/Format
Supplied as a liquid in 10mM Tris, pH 8.0, 5% BSA, 0.05% PEG, 0.02% sodium azide. Conjugated to Colloidal Gold.
Purity/Purification
Affinity Purified
Purified by GST-agarose affinity chromotography and p17 is cleaved from the fusion protein by using Factor Xa. GST is removed from the mixture by a second passage through GST-agarose.
Specificity
Gold conjugated p17 binds to murine monoclonal antibodies of defined epitope specificity and human polyclonal antibodies as determined by dot blot assays. Reaction time: 5-10 minutes at room temperature.
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.

Product Categories/Family for HIV-1 p17, Core, Strain IIIB recombinant protein

Molecular Biology; MB-Infectious Disease
Applications Tested/Suitable for HIV-1 p17, Core, Strain IIIB recombinant protein
Gel Shift Assay (GS/EMSA)
Application Notes for HIV-1 p17, Core, Strain IIIB recombinant protein
A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1:100 dilution range depending on the concentration of solid phase Anti-p17 in the test system. 2. Immunohistochemical studies may be performed in 1:50 dilution range.

Other Notes

Small volumes of HIV-1 p17, Core, Strain IIIB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIV-1 p17, Core, Strain IIIB purchase

MBS635570 is a recombinant protein targeting HIV-1 p17, Core, Strain IIIB. Also known as HIV-1 p17, Core, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Gold). Host: E Coli.
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