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NEK2, Active (NECAB3) Active Protein

Name: NECAB3 Active Protein; NEK2, Active; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS635709
Price: 1355 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635709

NEK2 is closely related in its catalytic domain to the Serine/Threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the NEK2 protein is… More details

$1355 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NEK2, active (NECAB3), Active Protein

Full Product Name

NEK2, active, Recombinant, Human (NIMA-Related Kinase 2, Never in mitosis A)

Product Gene Name

NECAB3 active protein

Form/Format

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity/Purification

Highly Purified
90% by densitometry

Preparation and Storage

This product is stable at -70 degree C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Molecular Weight

The approximate molecular weight is 76 kD (see Figure 1 below).

Chromosome Location

Chromosome: 20; NC_000020.10 (32244893..32262264, complement). Location: 20q11.22

OMIM

612478

3D Structure

ModBase 3D Structure for Q96P71

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Activity

The specific activity of NEK2 was determined to be 288 nmol/min/mg using a myelin basic protein (MBP) substrate (see activity assay protocol).

Product Categories/Family for NECAB3 active protein

Molecular Biology; MB-Protein Kinases

Other Notes

Small volumes of NECAB3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NECAB3 purchase

MBS635709 is an active protein targeting NECAB3. Also known as NEK2, active, Recombinant, Human (NIMA-Related Kinase 2, Never in mitosis A).

Additional Details

NCBI/Uniprot data below describe general gene information for NECAB3. It may not necessarily be applicable to this product.

NCBI GI #

149158725

NCBI GeneID

63941

NCBI Accession #

NP_112508.3

UniProt Primary Accession #

Q96P71

UniProt Secondary Accession #

Q5JWF5; Q5JWF6; Q5JWF7; Q86VV1; Q9H433; Q9H8G8; Q9HBW7; Q9HCQ9; A8K780; E1P5N2

UniProt Related Accession #

Q96P71

GenBank Nucleotide #

NM_031231.3

NCBI Official Full Name

N-terminal EF-hand calcium-binding protein 3 isoform 1

NCBI Official Synonym Full Names

N-terminal EF-hand calcium binding protein 3

NCBI Official Symbol

NECAB3

NCBI Official Synonym Symbols

NIP1; XB51; STIP3; EFCBP3; SYTIP2; APBA2BP; dJ63M2.4; dJ63M2.5

UniProt Protein Name

N-terminal EF-hand calcium-binding protein 3

UniProt Synonym Protein Names

Amyloid beta A4 protein-binding family A member 2-binding protein; Nek2-interacting protein 1; Neuronal calcium-binding protein 3; X11L-binding protein 51

Protein Family

N-terminal EF-hand calcium-binding protein

UniProt Gene Name

NECAB3

UniProt Synonym Gene Names

APBA2BP; NIP1; SYTIP2; XB51

UniProt Entry Name

NECA3_HUMAN

NCBI Protein Information

N-terminal EF-hand calcium-binding protein 3; X11L-binding protein 51; Nek2-interacting protein 1; EF-hand calcium binding protein 3; neuronal calcium-binding protein 3; synaptotagmin interacting protein 2; neuronal calcium-binding protein NECAB3; synaptotagmin interacting protein STIP3; amyloid beta A4 protein-binding family A member 2-binding protein; amyloid beta (A4) precursor protein-binding, family A, member 2 binding protein

NCBI Summary for NECAB3

The protein encoded by this gene interacts with the amino-terminal domain of the neuron-specific X11-like protein (X11L), inhibits the association of X11L with amyloid precursor protein through a non-competitive mechanism, and abolishes the suppression of beta-amyloid production by X11L. This protein, together with X11L, may play an important role in the regulatory system of amyloid precursor protein metabolism and beta-amyloid generation. The protein is phosphorylated by NIMA-related expressed kinase 2, and localizes to the Golgi apparatus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for NECAB3

NECAB3: Inhibits the interaction of APBA2 with beta-amyloid precursor protein (APP), and hence allows formation of beta- amyloid. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: endoplasmic reticulum membrane; cytoplasm; Golgi cis cisterna; nucleus

Molecular Function: protein binding; calcium ion binding

Biological Process: regulation of amyloid precursor protein biosynthetic process; protein metabolic process; protein secretion

Research Articles on NECAB3

1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with NECAB3 active protein

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>2 publications with NECAB3 and Nerve
Brain Antibodies	>2 publications with NECAB3 and Brain