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HIV-1 Env Gp120, Strain IIIB (HIV) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635758

MBS635758 is an active protein targeting HIV-1 env gp120, Strain IIIB. Also known as HIV-1 env gp120, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (FITC). More details

$890 / 50 Tests +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIV-1 env gp120, Strain IIIB, Active Protein
Full Product Name
HIV-1 env gp120, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (FITC)
Form/Format
Supplied as a liquid in 10mM Tris, pH 8.0. No stabilizers or preservatives added. Conjugated to FITC.
Purity/Purification
Affinity Purified
Purified by gel exclusion chromatography and UF concentrated.
Specificity
This FITC-conjugated gp120 binds to murine monoclonal antibodies of defined epitope specificity.
Preparation and Storage
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
This FITC-conjugated gp120 binds to CD4 bearing T-cells as determined by FACS.
Fluorochrome/Protein Ratio
4-5
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for HIV-1 env gp120, Strain IIIB active protein
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Gel Shift Assay (GS/EMSA)
Suitable for use in Direct Immunoflouorescent staining, FACS, Immunohistochemistry and Dot Blot.
Dilution: Direct Immunoflouorescence: 1:50
Dot Blot: 1:100 using purified anti-gp120 monoclonal or rabbit polyclonal antibodies.

Other Notes

Small volumes of HIV-1 env gp120, Strain IIIB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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