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Klotho Beta (KLB) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635769

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Klotho beta (KLB), Active Protein
Full Product Name
Klotho beta, Recombinant, Human (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)
Product Gene Name
KLB active protein
Form/Format
Supplied as a liquid in PBS, pH 6.8, 0.1mM EDTA, 50% glycerol.
Purity/Purification
Highly Purified
>95% by SDS-PAGE, reducing conditions and visualized with silver stain.
Concentration
0.27mg/ml (lot specific)
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
110kD
Chromosome Location
Chromosome: 4; NC_000004.11 (39408473..39453153). Location: 4p14
OMIM
611135
3D Structure
ModBase 3D Structure for Q86Z14
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Activity
Measured by its binding ability in a functional ELISA.
Endotoxin Level
<1EU/ug (LAL)
N-terminal Sequence Analysis
Phe53
Specific Activity
When Recombinant human FGF-21 is immobilized at 1ug/ml (100ul/well) Recombinant human Klotho B His-tag binds with an ED50 of 2.5-20ng/ml

Product Categories/Family for KLB active protein

Molecular Biology; MB-Enzymes; Glycosidase
Application Notes for KLB active protein
SDS-PAGE: 113-125kD, reducing conditions

Western Blot (WB) of KLB active protein

MBS635769 binds Recombinant Human FGF?21 in a functional ELISA. 50% of the optimal binding response for 200ng/well of recombinant human Klotho beta is observed at ~2.5-20ng/ml of recombinant human FGF-21.
KLB Active Protein – Cat# MBS635769

ELISA (EIA) of KLB active protein

Western Blot (reducing (R) conditions) analysis using MBS635769 (1ug/lane) and visualized by silver staining, showing a single band at 126kD.
KLB Active Protein, ELISA (EIA) – Cat# MBS635769

Other Notes

Small volumes of KLB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLB purchase

MBS635769 is an active protein targeting KLB. Also known as Klotho beta, Recombinant, Human (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein).

Additional Details

NCBI/Uniprot data below describe general gene information for KLB. It may not necessarily be applicable to this product.

NCBI GI #
119613331
NCBI GeneID
152831
NCBI Accession #
EAW92925.1
UniProt Primary Accession #
Q86Z14
UniProt Secondary Accession #
Q2M3K8
UniProt Related Accession #
Q86Z14
NCBI Official Full Name
klotho beta
NCBI Official Synonym Full Names
klotho beta
NCBI Official Symbol
KLB
NCBI Official Synonym Symbols
BKL
UniProt Protein Name
Beta-klotho
UniProt Synonym Protein Names
Klotho beta-like protein
Protein Family
Beta-klotho
UniProt Gene Name
KLB
UniProt Synonym Gene Names
BKL; BetaKlotho
UniProt Entry Name
KLOTB_HUMAN
NCBI Protein Information
beta-klotho; betaKlotho; klotho beta like; klotho beta-like protein

UniProt Comments for KLB

Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding; fibroblast growth factor binding; fibroblast growth factor receptor binding; hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of cell proliferation; carbohydrate metabolic process; insulin receptor signaling pathway; innate immune response
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Research Articles on KLB

1. betaKlotho suppresses tumor growth in hepatocellular carcinoma by regulating Akt/GSK-3beta/cyclin D1 signaling pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KLB active protein

 Reference Product  PubMed Publications
 FGF21 active protein  >23 publications with KLB and FGF21
 FGF19 active protein  >18 publications with KLB and FGF19
 FGFR4 active protein  >14 publications with KLB and FGFR4
 FGFR1 active protein  >12 publications with KLB and FGFR1
 FGF23 active protein  >5 publications with KLB and FGF23
 NR0B2 active protein  >1 publications with KLB and NR0B2
 CYP8B1 active protein  >1 publications with KLB and CYP8B1

Pathways associated with KLB active protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 FGFR Ligand Binding And Activation Pathway antibodies  FGFR Ligand Binding And Activation Pathway Diagram

Diseases associated with KLB active protein

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >5 publications with KLB and Liver Diseases
 Fatty Liver Antibodies  >5 publications with KLB and Fatty Liver
 Neoplasms Antibodies  >4 publications with KLB and Neoplasms
 Disease Models, Animal Antibodies  >4 publications with KLB and Disease Models, Animal
 Weight Loss Antibodies  >2 publications with KLB and Weight Loss
 Carcinoma, Hepatocellular Antibodies  >2 publications with KLB and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >2 publications with KLB and Liver Neoplasms
 Inflammation Antibodies  >2 publications with KLB and Inflammation
 Cardiovascular Diseases Antibodies  >1 publications with KLB and Cardiovascular Diseases
 Necrosis Antibodies  >1 publications with KLB and Necrosis

Organs/Tissues associated with KLB active protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >24 publications with KLB and Liver
 Adipose Tissue Antibodies  >13 publications with KLB and Adipose Tissue
 Brain Antibodies  >2 publications with KLB and Brain
 Eye Antibodies  >1 publications with KLB and Eye
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