Protein Kinase C Iota, Active (pkc-3) Active Protein

Name: pkc-3 Active Protein; MyBioSource
Brand: MyBioSource
SKU: MBS635833
Price: 670 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635833

N-terminal His6-tagged, recombinant full-length human PKC iota expressed in Sf21 cells. More details

$670 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Protein Kinase C iota, active (pkc-3), Active Protein

Full Product Name

Protein Kinase C iota, active, Recombinant, Human (PKCi)

Product Gene Name

pkc-3 active protein

Host

Sf21 cells

Form/Format

Supplied as a liquid in 20mM HEPES/NaOH, pH 7.4, 250mM sodium chloride, 50% glycerol, 5mM DTT, 2mM EDTA, 2mM EGTA, 0.05% Triton X-100.

Purity/Purification

Purified
~80% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose.

Preparation and Storage

Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Molecular Weight

67,842 Da

3D Structure

ModBase 3D Structure for A8WUG4

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Specific Activity

~800U/mg, where one unit of PKC iota activity is defined as 1nmol phosphate incorporated into 50uM PKCe Substrate Peptide (ERMRPRKRQGSVRRRV) per minute at 30 degree C with a final ATP concentration of 100uM.

Molecular Formula

68.6kD

Product Categories/Family for pkc-3 active protein

Molecular Biology; MB-Protein Kinases

Application Notes for pkc-3 active protein

Suitable for use in Kinase Assays.,
Dilution: Kinase Assay: 1.6-4.4ng of this lot of enzyme phosphorylated 50uM PKCtide Substrate Peptide in the assay. Assay background was subtracted from the actual counts to yield the results.

Other Notes

Small volumes of pkc-3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for pkc-3 purchase

MBS635833 is an active protein targeting pkc-3. Also known as Protein Kinase C iota, active, Recombinant, Human (PKCi). Host: Sf21 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for pkc-3. It may not necessarily be applicable to this product.

NCBI GI #

309371500

UniProt Primary Accession #

A8WUG4

NCBI Official Full Name

Protein CBR-PKC-3

UniProt Protein Name

Protein kinase C-like 3

UniProt Synonym Protein Names

Atypical protein kinase C-3

Protein Family

Protein kinase

UniProt Gene Name

pkc-3

UniProt Synonym Gene Names

aPKC3

UniProt Entry Name

KPC3_CAEBR

UniProt Comments for pkc-3

Function: Required for the normal progression of embryogenesis and viability of the organism. Plays an indispensable role in establishing embryonic polarity and in recruiting and maintaining par-6 to the periphery, through interaction with par-3. Required for epithelial cell polarity in the distal spermatheca. Phosphorylates serine residues of num-1

By similarity.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

By similarity.

Subunit structure: Interaction with par-3 required for the peripheral localization of par-6 and to form a par-3/par-6/pkc-3 complex, which is activated when cdc-42 interacts with par-6. Binds avidly to the phosphotyrosine interaction domain (PID) of a novel pkc-3 adapter protein num-1, which enables tethering and targeting of pkc-3 to the cell periphery

By similarity.

Subcellular location: Cytoplasm

By similarity. Cytoplasm › cytoskeleton

By similarity. Note: Targeted and anchored at the apical surface (villi) of intestinal and pharyngeal cells, and in proximity with the cortical actin cytoskeleton that lies under the plasma membrane. Tightly bound to organelles/cytoskeleton in six of the seven developmental stages. Accumulation in cytoplasm is restricted to L1 larvae and adults. Colocalized with par-3 at the anterior cortex of the 1-cell embryo

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 OPR domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.