• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Integrin Alpham, Beta2 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635841

DNA sequences encoding the extracellular domain of human Integrin alpha M (Phe17-Asn1105; Accession# P11215) (Corbi, A.L., et al., 1988, J. Biol. Chem. 263:12403) with an acidic tail at the C-terminus and the extracellular domain of human Integrin b2… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
Integrin alphaM, beta2, Active Protein
Full Product Name
Integrin alphaM, beta2, Recombinant, Human (Mac-1, CD11b/CD18)
Host
Chinese hamster ovary (CHO) cells
Form/Format
Supplied as lyophillized powder from 50mM Tris, 0.15M sodium chloride, 1mM MgCl2, pH7.4.
Purity/Purification
>95%, as determined by SDS-PAGE and visualized by silver stain.
Specificity
The 1088aa human aM/CD11b ECD shares 73-76% aa sequence identity with mouse, rat, bovine and canine aM while the 678aa human b2/CD18 ECD shares 81-83% aa sequence identity with mouse, rat, bovine, canine, goat, sheep, and porcine b2.
Preparation and Storage
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Activity
Measured by the ability of the immobilized protein to support the adhesion of CHO cells transfected with human ICAM-1. The ED50 for this effect is 0.25-1.5ug/ml.
Endotoxin
< 1EU/ug (LAL).
Reconstitution
Reconstitute with sterile PBS to 0.1mg/ml.
Molecular Mass
Based on N-terminal sequencing, the recombinant human Integrin aM subunit starts at Phe 17 and has a calculated molecular mass of ~124.7kD. The N-terminal sequence of the b2 subunit was blocked, suggesting that the b2 subunit may start at the predicted Gln 23 and has a calculated molecular mass of a ~79kD. As a result of glycosylation, the recombinant proteins migrate as ~165 and 100kD protein bands in SDS-PAGE under reducing conditions.

Product Categories/Family for Integrin alphaM, beta2 active protein

Molecular Biology; MB-Integrins

Other Notes

Small volumes of Integrin alphaM, beta2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Integrin alphaM, beta2 purchase

MBS635841 is an active protein targeting Integrin alphaM, beta2. Also known as Integrin alphaM, beta2, Recombinant, Human (Mac-1, CD11b/CD18). Host: Chinese hamster ovary (CHO) cells.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000