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MGMT, Active (MGMT) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635844

Many tumor promoting/causing agents (BCNU, CCNU, DTIC, procarbazine, temozolomide, etc) generate lethal cross-links at the O6-alkylguanine position in DNA. MGMT (O6-Methylguanine DNA Methyltransferase) removes alkyl adducts from the O6-position of guanine in… More details

$865 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MGMT, active, Active Protein
Full Product Name
MGMT, Recombinant, Human, active (O6-Methylguanine DNA Methyltransferase)
Product Gene Name
MGMT active protein
Form/Format
Supplied as a liquid in 20mM Tris, pH 7.5, 1mM DTT, 1mM EDTA, 200mM sodium chloride and 50% glycerol.
Purity/Purification
Highly Purified
0.9
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
21,646 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (131265454..131565783). Location: 10q26
OMIM
156569
3D Structure
ModBase 3D Structure for P16455
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
Minimum 10,000 Units/mg protein. One unit is defined as the amount of protein that cleaves 50% of 0.5 pmole of dsDNA oligomer substrate (O6-methylguanine) in 10 min at 37 degree C.

Product Categories/Family for MGMT active protein

Molecular Biology; MB-Enzymes; Methyltransferase

Other Notes

Small volumes of MGMT active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MGMT purchase

MBS635844 is an active protein targeting MGMT. Also known as MGMT, Recombinant, Human, active (O6-Methylguanine DNA Methyltransferase).

Additional Details

NCBI/Uniprot data below describe general gene information for MGMT. It may not necessarily be applicable to this product.

NCBI GI #
127069
NCBI GeneID
4255
NCBI Accession #
P16455.1
UniProt Primary Accession #
P16455
UniProt Secondary Accession #
Q5VY78
UniProt Related Accession #
P16455
NCBI Official Full Name
Methylated-DNA--protein-cysteine methyltransferase
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
MGMT
UniProt Protein Name
Methylated-DNA--protein-cysteine methyltransferase
UniProt Synonym Protein Names
6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Protein Family
Probable methylated-DNA--protein-cysteine methyltransferase
UniProt Gene Name
MGMT
UniProt Synonym Gene Names
MGMT
UniProt Entry Name
MGMT_HUMAN
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase

UniProt Comments for MGMT

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: DNA repair, damage; EC 2.1.1.63; Apoptosis; Methyltransferase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; DNA binding; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity

Biological Process: response to drug; DNA dealkylation; positive regulation of DNA repair; response to ethanol; response to folic acid; response to toxin; DNA methylation; DNA repair; regulation of caspase activity; DNA ligation
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Research Articles on MGMT

1. These findings suggest an alternative role for Gly131 and Gly132 in O-6-methylguanine-DNA-alkyltransferase: rendering the alpha-helix especially susceptible to perturbation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MGMT active protein

 Reference Product  PubMed Publications
 MLH1 active protein  >197 publications with MGMT and MLH1
 TP53 active protein  >93 publications with MGMT and TP53
 RASSF1 active protein  >79 publications with MGMT and RASSF1
 PTEN active protein  >72 publications with MGMT and PTEN
 MSH2 active protein  >55 publications with MGMT and MSH2
 ESR1 active protein  >27 publications with MGMT and ESR1
 APEX1 active protein  >12 publications with MGMT and APEX1
 DNMT3B active protein  >8 publications with MGMT and DNMT3B

Pathways associated with MGMT active protein

 Products by Pathway  Pathway Diagram
 DNA Damage Reversal Pathway antibodies  DNA Damage Reversal Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram

Organs/Tissues associated with MGMT active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >712 publications with MGMT and Brain
 Blood Antibodies  >219 publications with MGMT and Blood
 Lung Antibodies  >167 publications with MGMT and Lung
 Liver Antibodies  >114 publications with MGMT and Liver
 Bone Antibodies  >97 publications with MGMT and Bone
 Stomach Antibodies  >59 publications with MGMT and Stomach
 Lymph Antibodies  >47 publications with MGMT and Lymph
 Skin Antibodies  >46 publications with MGMT and Skin
 Intestine Antibodies  >45 publications with MGMT and Intestine
 Vascular Antibodies  >42 publications with MGMT and Vascular
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