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HIV-1 Tat, Strain IIIB (HIV) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635858

MBS635858 is a recombinant protein targeting HIV-1 tat, Strain IIIB. Also known as HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Gold). tat HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size). More details

$1325 / 1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIV-1 tat, Strain IIIB, Recombinant Protein
Full Product Name
HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Gold)
Form/Format
Supplied as a liquid in 10mM Tris, pH 8.0, 5% BSA, 0.05% PEG, 0.02% sodium azide. Conjugated to Colloidal Gold.
Purity/Purification
Affinity Purified
Purified by ion affinity and reverse phase HPLC to 99% as determined by SDS-PAGE and HPLC.
Specificity
Gold conjugated tat binds to murine monoclonal antibodies of defined epitope specificity and human/rabbit polyclonal antibodies as determined by dot blot assays.
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.
Applications Tested/Suitable for HIV-1 tat, Strain IIIB recombinant protein
Gel Shift Assay (GS/EMSA)
A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1:100 dilution range depending on the concentration of solid phase Anti-tat in the test system. 2. Immunohistochemical studies may be performed in 1:50 dilution range.

Other Notes

Small volumes of HIV-1 tat, Strain IIIB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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