• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

UBC9 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635888

Human UBC9 Ubiquitin-like protein SUMO-1 conjugating enzyme is also called UBE2I. rHuUBC9 Mediates the conjugation of the ubiquitin-like protein SUMO-1 to a variety of proteins including RanGAP1, IkappaB alpha, and PML without the requirement of an ubiquitin… More details

$750 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ubc9, Active Protein
Full Product Name
Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9)
Product Gene Name
Host
E Coli
Form/Format
Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 100mM sodium chloride, 100mM imidazole, 0.5mM PMSF, 1mM DTT, 10% glycerol.
Purity/Purification
Highly Purified
95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
17,911 Da
3D Structure
ModBase 3D Structure for P50623
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by H-thymidine uptake) is less then 1.0ng/ml, corresponding to a specific activity of 1x10e6 U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20ng/ml.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of ubiquitin-conjugating enzyme
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm
2. Analysis by RP-HPLC, using a standard solution of UBC-9 as a Reference Standard.

Product Categories/Family for UBC9 active protein

Other Notes

Small volumes of UBC9 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBC9 purchase

MBS635888 is an active protein targeting UBC9. Also known as Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for UBC9. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
UBC9
UniProt Protein Name
SUMO-conjugating enzyme UBC9
UniProt Synonym Protein Names
Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase
UniProt Gene Name
UBC9
UniProt Entry Name
UBC9_YEAST

UniProt Comments for UBC9

Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5

Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathway: Protein modification; protein sumoylation.

Subunit structure: Interacts with SIZ1. Ref.5 Ref.6

Subcellular location: Nucleus Ref.7.

Miscellaneous: Present with 2600 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000