CDC34 Recombinant Protein

Name: CDC34 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS635890
Price: 680 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635890

UbcH3 also called Cdc34 is important in the control of cell cycle and DNA replication. Cdc34 in association with different E3 complexes, including SCF, has been shown to target many different substrates for ubiquitination and degradation during cell division,… More details

$680 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

cdc34, Recombinant Protein

Full Product Name

cdc34, Recombinant, Human (Ubc3, Ubiquitin Conjugating Enzyme)

Product Gene Name

CDC34 recombinant protein

Sequence

MHHHHHHAMG ILMARPLVPS SQKALLLELK GLQEEPVEGF RVTLVDEGDL YNWEVAIFGP PNTYYEGGYF KARLKF PIDYPYSPPA FRFLTKMWHP NIYETGDVCI SILHPPVDDP QSGELPSERW NPTQNVRTIL LSVISLLNEP NTFSPAN VDASVMYRKW KESKGKDREY TDIIRKQVTK VDAERDGVKV PTTLAEYCVK TKAPAPDEGS DLFYDDYYED GEV EEEADSCFGD DEDDSGTEES .

Form/Format

Supplied as a lyophilized powder in 1X PBS, 1mM DTT, pH 7.5.

Purity/Purification

Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

Preparation and Storage

-20 degree C
Stability: Lyophilized Recombinant Human CDC34 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Recombinant CDC34 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight

28kD

3D Structure

ModBase 3D Structure for P14682

Endotoxin Level

0.1ng/ug (IEU/ug) of Human Ubc3.

Solubility

It is recommended to reconstitute the lyophilized Human Ubc3 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for CDC34 recombinant protein

Molecular Biology; MB-Enzymes

Other Notes

Small volumes of CDC34 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC34 purchase

MBS635890 is a recombinant protein targeting CDC34. Also known as cdc34, Recombinant, Human (Ubc3, Ubiquitin Conjugating Enzyme).

Additional Details

NCBI/Uniprot data below describe general gene information for CDC34. It may not necessarily be applicable to this product.

NCBI GI #

1431497

NCBI Accession #

CAA98872.1

UniProt Primary Accession #

P14682

UniProt Secondary Accession #

D6VS40

NCBI Official Full Name

CDC34

UniProt Protein Name

Ubiquitin-conjugating enzyme E2-34 kDa

UniProt Synonym Protein Names

Cell division control protein 34; E3 ubiquitin ligase complex SCF subunit CDC34; Ubiquitin carrier protein; Ubiquitin-protein ligase

Protein Family

Ubiquitin-conjugating enzyme

UniProt Gene Name

CDC34

UniProt Synonym Gene Names

DNA6; UBC3

UniProt Entry Name

UBC3_YEAST

UniProt Comments for CDC34

Function: Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A. Ref.1 Ref.5 Ref.6Mediates the initiation of DNA replication (transition of G1 to S phase in cell cycle). Essential component of the E3 ubiquitin ligase complex SCF (SKP1-CUL1-F-box protein), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the regulation of methionine biosynthesis genes and in the degradation of CDC6 together with CDC4 and CDC53. Ref.1 Ref.5 Ref.6

Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with CDC53. Component of the E3 ubiquitin ligase complexes SCF with CDC53, SKP1/CBF3D, HRT1 and some F-box proteins like MET30 and CDC4. Ref.6 Ref.7

Subcellular location: Cytoplasm. Nucleus Ref.8.

Domain: The acidic C-terminal extension is essential for the cell cycle function.

Miscellaneous: Present with 8170 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CDC34 recombinant protein

Reference Product	PubMed Publications
SKP1 recombinant protein	>44 publications with CDC34 and SKP1
SIC1 recombinant protein	>31 publications with CDC34 and SIC1
CDC53 recombinant protein	>30 publications with CDC34 and CDC53
CDC4 recombinant protein	>29 publications with CDC34 and CDC4
GRR1 recombinant protein	>11 publications with CDC34 and GRR1
MET4 recombinant protein	>7 publications with CDC34 and MET4
MET30 recombinant protein	>6 publications with CDC34 and MET30
HRT1 recombinant protein	>5 publications with CDC34 and HRT1
RTT101 recombinant protein	>1 publications with CDC34 and RTT101
UBI4 recombinant protein	>1 publications with CDC34 and UBI4