Membrane Frizzled-Related Protein (MFRP) Active Protein

Name: MFRP Active Protein; MyBioSource
Brand: MyBioSource
SKU: MBS635899
Price: 1260 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635899

MFRP (membrane-type frizzled-related protein) is a 65kD, type II transmembrane protein related to both Tolloid proteases and frizzled-domain containing Wnt pathway proteins (1-4). Human MFRP is 579 amino acids (aa) in length (3, 5, 6). It contains a 69aa… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Membrane Frizzled-Related Protein (MFRP), Active Protein

Full Product Name

Membrane Frizzled-Related Protein, Recombinant, Human (Membrane-type Frizzled-Related Protein, MFRP)

Product Gene Name

MFRP active protein

Form/Format

Supplied as a lyophilized powder in PBS.

Purity/Purification

Highly Purified
90%, as determined by SDS-PAGE and visualized by silver stain.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

Based on N-terminal amino acid sequencing, the recombinant human MFRP begins with the histidine tag. It has a predicted molecular mass of approximately 52.7kDa As a result of glycosylation, the recombinant monomer migrates as an approximately 90-105kD pro

Chromosome Location

Chromosome: 11; NC_000011.9 (119209644..119217383, complement). Location: 11q23

OMIM

606227

3D Structure

ModBase 3D Structure for Q9BY79

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Activity

Measured by its ability to inhibit the activity of rhBMP-1 on the cleavage of a fluorogenic peptide substrate, Mca-Y-V-A-D-A-P-K(Dnp)-OH. Cleavage of ES007 can be measured using excitation and emission wavelengths of 320nm and 405nm, respectively. 30ug/ml of rhMFRP reduces rhBMP-1 activity by 30-60% when measured with 1ug of BMP-1 and 10uM ES007 in 100ul of 10 mM HEPES, 0.01% Brij 35, pH 7.5 at RT.

Product Categories/Family for MFRP active protein

Molecular Biology; MB-Wnt Proteins

Other Notes

Small volumes of MFRP active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MFRP purchase

MBS635899 is an active protein targeting MFRP. Also known as Membrane Frizzled-Related Protein, Recombinant, Human (Membrane-type Frizzled-Related Protein, MFRP).

Additional Details

NCBI/Uniprot data below describe general gene information for MFRP. It may not necessarily be applicable to this product.

NCBI GI #

13899255

NCBI GeneID

83552

NCBI Accession #

NP_113621.1

UniProt Primary Accession #

Q9BY79

UniProt Secondary Accession #

Q335M3; Q96DQ9; B0YJ36; B0YJ37

UniProt Related Accession #

Q9BY79

GenBank Nucleotide #

NM_031433.3

NCBI Official Full Name

membrane frizzled-related protein

NCBI Official Synonym Full Names

membrane frizzled-related protein

NCBI Official Symbol

MFRP

NCBI Official Synonym Symbols

RD6; NNO2; MCOP5

UniProt Protein Name

Membrane frizzled-related protein

UniProt Synonym Protein Names

Membrane-type frizzled-related protein

Protein Family

Membrane frizzled-related protein

UniProt Gene Name

MFRP

UniProt Entry Name

MFRP_HUMAN

NCBI Protein Information

membrane frizzled-related protein; membrane-type frizzled-related protein

NCBI Summary for MFRP

This gene encodes a member of the frizzled-related protein family. The encoded protein plays an important role in eye development and mutations in this gene have been associated with nanophthalmos, posterior microphthalmia, retinitis pigmentosa, foveoschisis, and optic disc drusen. The protein is encoded by a bicistronic transcript which also encodes C1q and tumor necrosis factor related protein 5 (C1QTNF5). [provided by RefSeq, Jun 2013]

UniProt Comments for MFRP

MFRP: May play a role in eye development. Defects in MFRP are the cause of nanophthalmos 2 (NNO2). NNO2 is a rare autosomal recessive disorder of eye development characterized by extreme hyperopia and small functional eyes. Defects in MFRP are the cause of microphthalmia isolated type 5 (MCOP5). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. MCOP5 is characterized by posterior microphthalmia, retinitis pigmentosa, foveoschisis and optic disc drusen.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: apical plasma membrane; integral to membrane

Biological Process: embryonic development

Disease: Microphthalmia, Isolated 5; Nanophthalmos 2

Research Articles on MFRP

1. Among five single nucleotide polymorphism(SNP)s tested, only MFRP rs3814762 and heat shock protein (HSP)70 rs1043618 show nominal association with primary angle closure.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MFRP active protein

Reference Product	PubMed Publications
C1QTNF5 active protein	>6 publications with MFRP and C1QTNF5
NR2E3 active protein	>1 publications with MFRP and NR2E3
VSX2 active protein	>1 publications with MFRP and VSX2

Diseases associated with MFRP active protein

Disease Name	Pubmed Publications
Congenital Abnormalities Antibodies	>17 publications with MFRP and Congenital Abnormalities
Eye Abnormalities Antibodies	>17 publications with MFRP and Eye Abnormalities
Macular Degeneration Antibodies	>6 publications with MFRP and Macular Degeneration
Atrophy Antibodies	>3 publications with MFRP and Atrophy
Disease Models, Animal Antibodies	>2 publications with MFRP and Disease Models, Animal
Edema Antibodies	>2 publications with MFRP and Edema
Necrosis Antibodies	>2 publications with MFRP and Necrosis
Craniofacial Abnormalities Antibodies	>1 publications with MFRP and Craniofacial Abnormalities
Lens Diseases Antibodies	>1 publications with MFRP and Lens Diseases
Skin Neoplasms Antibodies	>1 publications with MFRP and Skin Neoplasms

Organs/Tissues associated with MFRP active protein

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>33 publications with MFRP and Eye
Brain Antibodies	>4 publications with MFRP and Brain
Muscle Antibodies	>3 publications with MFRP and Muscle
Prostate Antibodies	>2 publications with MFRP and Prostate
Skin Antibodies	>2 publications with MFRP and Skin
Vascular Antibodies	>1 publications with MFRP and Vascular
Ovary Antibodies	>1 publications with MFRP and Ovary
Uterus Antibodies	>1 publications with MFRP and Uterus
Stomach Antibodies	>1 publications with MFRP and Stomach
Lung Antibodies	>1 publications with MFRP and Lung