Human TNFSF10 Active Protein – Cat# MBS635917

TRAIL (TNFSF10) Human Active Protein

Name: TNFSF10 Active Protein; TRAIL; Human; MyBioSource
Brand: MyBioSource
SKU: MBS635917
Price: 790 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635917

TRAIL, a member of the TNF ligand superfamily, induces apoptosis in tumor cells but not normal cells. TRAIL is expressed by the cells of many tissues, including thymus, spleen, lung, kidney and others. This recombinant protein corresponds to the TNF-like… More details

$790 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TRAIL (TNFSF10), Active Protein

Full Product Name

TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)

Product Gene Name

TNFSF10 active protein

Host

E Coli

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.2, 110mM sodium chloride. Sterile filtered prior to lyophilization.

Purity/Purification

Highly Purified
98% by SDS PAGE and HPLC analyses. Purified by sequential chromatography.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, briefly centrifuge the original vial prior to removing the cap.

Molecular Weight

19.6kD

Chromosome Location

Chromosome: 3; NC_000003.11 (172223298..172241297, complement). Location: 3q26

OMIM

603598

3D Structure

ModBase 3D Structure for P50591

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

ED50 = 1-3ng/ml, determined by the dose dependent induction of apoptosis with U343MG cells.
Reconstitution Reconstitute in sterile, dH2O to 0.5-1.0mg/ml. Further dilutions should be made in aqueous buffers with carrier protein, such as 0.1-1.0% BSA.

Endotoxin Level

0.1ng/ug

Product Categories/Family for TNFSF10 active protein

Molecular Biology; MB-Apoptosis

Other Notes

Small volumes of TNFSF10 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNFSF10 purchase

MBS635917 is an active protein targeting TNFSF10. Also known as TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10). Reactive in Human. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TNFSF10. It may not necessarily be applicable to this product.

NCBI GI #

4507593

NCBI GeneID

8743

NCBI Accession #

NP_003801.1

UniProt Primary Accession #

P50591

UniProt Secondary Accession #

A1Y9B3

UniProt Related Accession #

P50591

GenBank Nucleotide #

NM_003810.3

NCBI Official Full Name

tumor necrosis factor ligand superfamily member 10 isoform 1

NCBI Official Synonym Full Names

tumor necrosis factor (ligand) superfamily, member 10

NCBI Official Symbol

TNFSF10

NCBI Official Synonym Symbols

TL2; APO2L; CD253; TRAIL; Apo-2L

UniProt Protein Name

Tumor necrosis factor ligand superfamily member 10

UniProt Synonym Protein Names

Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand

Protein Family

Tumor necrosis factor ligand superfamily

UniProt Gene Name

TNFSF10

UniProt Synonym Gene Names

APO2L; TRAIL; Apo-2L

UniProt Entry Name

TNF10_HUMAN

NCBI Protein Information

tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta

NCBI Summary for TNFSF10

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

UniProt Comments for TNFSF10

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: extracellular space; integral to plasma membrane; extracellular region

Molecular Function: protein binding; metal ion binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; cell-cell signaling; apoptosis; positive regulation of apoptosis; male gonad development; positive regulation of caspase activity; immune response; signal transduction; response to insulin stimulus

Research Articles on TNFSF10

1. SIRT1 inhibition increases Ku70 acetylation, and the acetylated Ku70 with a decreased function mediates the induction of DR5 and the down-regulation of c-FLIP by up-regulating c-Myc/ATF4/CHOP pathway,promotes the TRAIL-induced apoptosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TNFSF10 active protein

Reference Product	PubMed Publications
TNFRSF10B active protein	>2564 publications with TNFSF10 and TNFRSF10B
CASP8 active protein	>1228 publications with TNFSF10 and CASP8
FADD active protein	>590 publications with TNFSF10 and FADD
TNFRSF10A active protein	>531 publications with TNFSF10 and TNFRSF10A
CFLAR active protein	>241 publications with TNFSF10 and CFLAR
TNFRSF11B active protein	>163 publications with TNFSF10 and TNFRSF11B
TNFRSF10C active protein	>107 publications with TNFSF10 and TNFRSF10C
CASP10 active protein	>61 publications with TNFSF10 and CASP10
TRADD active protein	>57 publications with TNFSF10 and TRADD
TNFRSF10D active protein	>49 publications with TNFSF10 and TNFRSF10D

Pathways associated with TNFSF10 active protein

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
Caspase-8 Activation Pathway antibodies	Caspase-8 Activation Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Death Receptor Signalling Pathway antibodies	Death Receptor Signalling Pathway Diagram
Dimerization Of Procaspase-8 Pathway antibodies	Dimerization Of Procaspase-8 Pathway Diagram

Diseases associated with TNFSF10 active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>3597 publications with TNFSF10 and Necrosis
Neoplasms Antibodies	>2895 publications with TNFSF10 and Neoplasms
Leukemia Antibodies	>432 publications with TNFSF10 and Leukemia
Colorectal Neoplasms Antibodies	>314 publications with TNFSF10 and Colorectal Neoplasms
Liver Diseases Antibodies	>310 publications with TNFSF10 and Liver Diseases
Breast Neoplasms Antibodies	>283 publications with TNFSF10 and Breast Neoplasms
Liver Neoplasms Antibodies	>268 publications with TNFSF10 and Liver Neoplasms
Neoplasms, Experimental Antibodies	>255 publications with TNFSF10 and Neoplasms, Experimental
Lung Neoplasms Antibodies	>248 publications with TNFSF10 and Lung Neoplasms
Glioma Antibodies	>227 publications with TNFSF10 and Glioma

Organs/Tissues associated with TNFSF10 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1010 publications with TNFSF10 and Blood
Liver Antibodies	>412 publications with TNFSF10 and Liver
Lung Antibodies	>340 publications with TNFSF10 and Lung
Prostate Antibodies	>301 publications with TNFSF10 and Prostate
Brain Antibodies	>208 publications with TNFSF10 and Brain
Vascular Antibodies	>171 publications with TNFSF10 and Vascular
Kidney Antibodies	>135 publications with TNFSF10 and Kidney
Skin Antibodies	>119 publications with TNFSF10 and Skin
Bone Marrow Antibodies	>97 publications with TNFSF10 and Bone Marrow
Muscle Antibodies	>72 publications with TNFSF10 and Muscle