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MAPKAP Kinase 2, Active (MAPKAPK2) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635986

N-terminal, GST-tagged, fusion protein encoding amino acids 46-400 of human MAPKAP-K2; expressed in E. coli. More details

$1375 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAPKAP Kinase 2, active (Mapkapk2), Active Protein
Full Product Name
MAPKAP Kinase 2, active, Recombinant, Human (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2)
Product Gene Name
Mapkapk2 active protein
Host
E Coli
Form/Format
Supplied as 5ug of enzyme in 50ul of Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 1mM benzamidine, 5mM dithiothreitol, 50% glycerol.
Purity/Purification
Highly Purified
~80% by SDS-PAGE. Purified using glutathione-agarose. Activated with MAPK and re-purified by Q-Sepharose chromatography.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
66kD
Chromosome Location
Chromosome: 13; NC_005112.3 (52865897..52912481, complement). Location: 13q13
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
~2000 U/mg.
Unit Definition
1 unit of MAPKAP kinase 2 activity is defined as 1nmol phosphate incorporated into 30uM substrate peptide (KKLNRTLSVA), per minute at 30 degree C with a final ATP concentration of 100uM..

Product Categories/Family for Mapkapk2 active protein

Molecular Biology; MB-Protein Kinases

SDS-Page of Mapkapk2 active protein

SDS-PAGE and Coomassie Stain: Purity was assessed by|SDS-PAGE and Coomassie blue staining using 3ug of M2355-05.
MAPKAPK2 Active Protein – Cat# MBS635986

Testing Data of Mapkapk2 active protein

Sample Activity data. 0.7–5.7ng of enzyme phosphorylated 30uM substrate peptide (KKLNRTLSVA) in the assay described in protocol. Assay background was subtracted from the actual counts to yield the results shown below.
MAPKAPK2 Active Protein, Testing Data – Cat# MBS635986

Other Notes

Small volumes of Mapkapk2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mapkapk2 purchase

MBS635986 is an active protein targeting Mapkapk2. Also known as MAPKAP Kinase 2, active, Recombinant, Human (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Mapkapk2. It may not necessarily be applicable to this product.

NCBI GI #
30017431
NCBI GeneID
289014
NCBI Accession #
NP_835203.1
GenBank Nucleotide #
NM_178102.2
NCBI Official Full Name
MAP kinase-activated protein kinase 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase-activated protein kinase 2
NCBI Official Symbol
Mapkapk2
NCBI Protein Information
MAP kinase-activated protein kinase 2

NCBI Summary for Mapkapk2

mediates formation of actin stress fibers in septal neuronal cells [RGD, Feb 2006]

Research Articles on Mapkapk2

1. MK2 small interfering RNA delivery arrests lipopolysaccharide-induced inflammatory bone loss, decreases inflammatory infiltrate, and decreases osteoclastogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Mapkapk2 active protein

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 Destabilization Of MRNA By Tristetraprolin (TTP) Pathway antibodies  Destabilization Of MRNA By Tristetraprolin (TTP) Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 IL-2 Signaling Pathway antibodies  IL-2 Signaling Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
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