Listeriolysin O, PEST-Free Active Protein

The secreted pore-forming protein listeriolysin O (LLO) is an essential virulence determinant of the facultative intracellular bacterium Listeria monocytogenes that allows the pathogen to escape from the phagocytic vacuole and reach the host cytosol. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. At its NH2-terminus it possesses a 25 residues long typical signal sequence excited during the secretion process. Moreover, in its NH2-terminus there is also a 19 amino acids PEST-like sequence that may target this toxin for degradation. This motif seems to be essential for bacterial virulence too, since its deletion causes a defect in bacterium phagosomal escaping.

Recombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence. LLO-PEST minus is composed of 471 amino acids, starting from amino acid 60 to amino acid 529, with the addition of a methionine in its NH2-terminus.

Small volumes of Listeriolysin O, PEST-free active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS636027 is an active protein targeting Listeriolysin O, PEST-free. Also known as Listeriolysin O, PEST-free, Recombinant (LLO). Host: E Coli.