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UFC1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6361094

Various ubiquitin-like (UBL) post-translational modifiers contribute critically to the protein degradation pathway. These UBLs are covalently linked to most target proteins through an enzymatic cascade analogous to ubiquitylation, consisting of E1… More details

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Product Overview

Online Datasheet in PDF
Product Name
Ufc1, Polyclonal Antibody
Full Product Name
Ufc1, CT (UFC1, Ubiquitin-fold modifier-conjugating enzyme 1) (FITC)
Product Gene Name
Product Synonym Names
UFC1; Ubiquitin-fold modifier-conjugating enzyme 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
19,458 Da
OMIM
610554
3D Structure
ModBase 3D Structure for Q9Y3C8
Immunogen
Ufc1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Ufc1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-Ufc1 antibody

Applications Tested/Suitable for anti-Ufc1 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-Ufc1 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-Ufc1 antibody

Ufc1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Ufc1 antibody detected the Ufc1 protein (arrow).
Rabbit Anti-UFC1 Polyclonal Antibody – Cat# MBS6361094

Western Blot (WB) of anti-Ufc1 antibody

Western blot analysis of Ufc1 (arrow) using rabbit polyclonal Ufc1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ufc1 gene.
Rabbit Anti-UFC1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6361094

Immunohistochemistry (IHC) of anti-Ufc1 antibody

Ufc1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Ufc1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-UFC1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6361094

Flow Cytometry (FC/FACS) of anti-Ufc1 antibody

Ufc1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-UFC1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6361094

Product References and Citations for anti-Ufc1 antibody

Komatsu, M., et al., EMBO J. 23(9):1977-1986 (2004).

Other Notes

Small volumes of anti-Ufc1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ufc1 purchase

MBS6361094 is an antibody targeting Ufc1. Also known as Ufc1, CT (UFC1, Ubiquitin-fold modifier-conjugating enzyme 1) (FITC). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Ufc1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-fold modifier-conjugating enzyme 1
NCBI Official Synonym Full Names
ubiquitin-fold modifier conjugating enzyme 1
NCBI Official Symbol
UFC1
NCBI Official Synonym Symbols
HSPC155
UniProt Protein Name
Ubiquitin-fold modifier-conjugating enzyme 1
UniProt Gene Name
UFC1
UniProt Synonym Gene Names
Ufm1-conjugating enzyme 1
UniProt Entry Name
UFC1_HUMAN
NCBI Protein Information
ubiquitin-fold modifier-conjugating enzyme 1; Ufm1-conjugating enzyme 1

NCBI Summary for Ufc1

UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Mar 2008]

UniProt Comments for Ufc1

UFC1: E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage. Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q23.3

Molecular Function: protein binding

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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