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VILL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6362576

The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq]. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
VILL, Polyclonal Antibody
Full Product Name
VILL, ID (VILL, Villin-like protein) (AP)
Product Gene Name
Product Synonym Names
VILL; Villin-like protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human VILL.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
95,907 Da
3D Structure
ModBase 3D Structure for O15195
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 385-412 in the central region of human VILL, UniProt Accession #NP_056957.3; O15195.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-VILL antibody

Applications Tested/Suitable for anti-VILL antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-VILL antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-VILL antibody

Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS649681. This demonstrates that MBS649681 detected the VILL protein (arrow).
Rabbit Anti-VILL Polyclonal Antibody – Cat# MBS6362576

Immunohistochemistry (IHC) of anti-VILL antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS649681 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649681 for immunohistochemistry.
Rabbit Anti-VILL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6362576

Product References and Citations for anti-VILL antibody

1. Piana, S., et al. J. Mol. Biol. 375(2):460-470(2008). 2. Yamamichi, N., et al. Cancer Res. 67(22):10727-10735(2007). 3. Tomar, A., et al. J. Biol. Chem. 281(42):31972-31986(2006). 4. Ishikawa, S., et al. DNA Res. 4(1):35-43(1997).

Other Notes

Small volumes of anti-VILL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VILL purchase

MBS6362576 is an antibody targeting VILL. Also known as VILL, ID (VILL, Villin-like protein) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VILL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
villin-like protein
NCBI Official Synonym Full Names
villin-like
NCBI Official Symbol
VILL
UniProt Protein Name
Villin-like protein
Protein Family
UniProt Gene Name
VILL
UniProt Entry Name
VILL_HUMAN
NCBI Protein Information
villin-like protein

NCBI Summary for VILL

The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq, Jul 2008]

UniProt Comments for VILL

Function: Possible tumor suppressor.

Tissue specificity: Ubiquitously expressed in 16 tissues examined.

Sequence similarities: Belongs to the villin/gelsolin family.Contains 6 gelsolin-like repeats.Contains 1 HP (headpiece) domain.

Sequence caution: The sequence AAH00243.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH04300.1 differs from that shown. Reason: Erroneous initiation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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