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CD152, Fc Chimera (CD152) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636307

CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity… More details

$750 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD152, Fc Chimera, Active Protein
Full Product Name
CD152, Fc Chimera, Recombinant, Human (CTLA4, Cytotoxic T-lymphocyte-associated Antigen 4)
Sequence
The sequence of the amino acids was determined and was found to be: Lys-Ala-Me t-His-Val.
Host
Sf9 Insect cells
Form/Format
Supplied as a lyophilized powder without additives. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. to a concentration of >0.1mg/ml. This solution can then be diluted into other aqueous solutions.
Purity/Purification
Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug) of CTLA-4.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA. aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight
80kD
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Recombinant CTLA-4 is fully biologically active when compared to standards.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280.
2. Analysis by RP-HPLC, using a standard solution of Human CTLA-4 as a Reference Standard.

Product Categories/Family for CD152, Fc Chimera active protein

Molecular Biology; MB-CD Markers

Other Notes

Small volumes of CD152, Fc Chimera active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD152, Fc Chimera purchase

MBS636307 is an active protein targeting CD152, Fc Chimera. Also known as CD152, Fc Chimera, Recombinant, Human (CTLA4, Cytotoxic T-lymphocyte-associated Antigen 4). Host: Sf9 Insect cells.
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