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Staphylokinase (sak) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636386

Recombinant Human Staphylokinase is a non-glycosylated polypeptide chain having a molecular weight of 15.5kD. More details

$680 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Staphylokinase (sak), Active Protein
Full Product Name
Staphylokinase, Recombinant, Human
Product Gene Name
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ser-Se r-Phe-Asp.
Host
E Coli
Form/Format
Supplied as a lyophilized powder in 20mM phosphate buffer, pH 7.0.
Purity/Purification
Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
18,520 Da
Chromosome Location
NC_002745.2 (2009086..2009577, complement)
3D Structure
ModBase 3D Structure for Q99SU7
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Staphylokinase is fully biologically active when compared to standard. The biological activity measured by the ability of fibrin lysis in agarose plate was found to be ~4600IU/mg.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of Human Staphylokinase.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Staphylokinase as a Reference Standard.
Reconstitution
Reconstitute the lyophilized Human SAK in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for sak active protein

Other Notes

Small volumes of sak active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for sak purchase

MBS636386 is an active protein targeting sak. Also known as Staphylokinase, Recombinant, Human. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for sak. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
staphylokinase
NCBI Official Symbol
sak
UniProt Protein Name
Staphylokinase
Protein Family
UniProt Gene Name
sak
UniProt Entry Name
SAK_STAAN
NCBI Protein Information
staphylokinase

UniProt Comments for sak

Function: Potent plasminogen activator that converts plasminogen into plasmin. It forms a 1:1 complex with plasmin, which in turn activates other plasminogen molecules

By similarity.

Subcellular location: Secreted

By similarity.

Sequence similarities: Belongs to the staphylokinase family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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