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Ubc12 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636425

Recombinant Human UbcH12 is functional in in vitro NEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APP-BP1 binds to the amyloid precursor protein (APP)… More details

$750 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ubc12, Recombinant Protein
Full Product Name
Ubc12, Recombinant, Human (Ubiquitin Conjugating Enzyme 12)
Sequence
MSYYHHHHHH DYDIPTTENL YFQGAMDPEF RIWMIKLFSL KQQKKEEESA GGTKGSSKKA SAAQLRIQKD INE LNLPKTCDIS FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNIL REDWKPVLTI NSIIYGLQYL FLEPNPEDPL NKEAVLQNNR RLFEQNVQRS MRGGYIGSTY FERCLK.
Host
E Coli
Form/Format
Supplied as a lyophilized powder in 1X PBS, 1mM DTT, pH 7.5.
Purity/Purification
Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Preparation and Storage
-20 degree C
Stability: Lyophilized rHuUbc12 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rhUbc12 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight
25kD
Endotoxin Level
0.1ng/ug (IEU/ug) of rHuUbc12.
Solubility
It is recommended to reconstitute the lyophilized rHuUbc12 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Other Notes

Small volumes of Ubc12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ubc12 purchase

MBS636425 is a recombinant protein targeting Ubc12. Also known as Ubc12, Recombinant, Human (Ubiquitin Conjugating Enzyme 12). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Ubc12. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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