Phosphoinositide 3-Kinase Gamma, P110 Gamma, Catalytically Inactive Mutant Recombinant Protein

The PI3K D946A protein is a catalytically inactive mutant of PI3K with a D946A mutation in the ATP binding site. This recombinant catalytically inactive protein can be used as a negative control in any kind of PI3K kinase activity studies. Recombinant full length PI3K DA mutant carries a N-terminal GST-Tag and was purified by affinity chromatography. The GST-Tag facilitates the protein's application in typical GST pull-down assays.

Phosphoinositide 3-kinases (PI3Ks) phosphorylate (PIs) at their 3´-OH position generating lipid second messengers and thereby regulate numeroMyBioSource processes including cell growth,differentiation, survival, proliferation, migration and metabolism. On the basis of structural similarities and substrate specificity, the PI3K family can be subdivided into three classes termed I, II, and III. All human class I members are heterodimers consisting of a catalytic subunit (MW approx. 110kD) and a noncatalytic subunit (MW 50, 55, 85, or 101kD) and are known tophosphorylate phosphatidylinositol (PI), phosphatidylinositol-4-mono-phosphate (PIP) andphosphatidylinositol-4,5-bisphosphate (PIP2) in vitro. The class I members can be further subdivided into class IA and IB PI3Ks. Class IA exists in three isoforms (p110, p110 and p110) whereas the only class IB member is termed p110. Class IA PI3Ks are activated by adaptorproteins such as Ras or BCAP, or tyrosine-kinase-associated receptors including antigen, co-stimulatory and cytokine receptors (e.g. CD19, CD28, Insulin receptor, EGFR, and PDGFR). p110 is activated by G-protein-coupled receptors (GPCRs). Effectors of class I PI3Ks are pleckstrin-homology domain proteins such as Akt/PKB, BTK, TEC, ITK, BAM32, and small GTPases (e.g. Cdc42, Rac, or Ras). The action of PI3Ks is regulated by the phosphatidylinositol- 3,4,5-trisphosphate phosphatases SHIP and PTEN.

Small volumes of Phosphoinositide 3-kinase gamma, p110 gamma, catalytically inactive mutant recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS636549 is a recombinant protein targeting Phosphoinositide 3-kinase gamma, p110 gamma, catalytically inactive mutant. Also known as Phosphoinositide 3-kinase gamma, p110 gamma, catalytically inactive mutant, Recombinant, Human (PI3K).