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HIV-1 Tat, Strain IIIB (HIV) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636668

MBS636668 is an active protein targeting HIV-1 tat, Strain IIIB. Also known as HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin). Host: E Coli. More details

$1000 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIV-1 tat, Strain IIIB, Active Protein
Full Product Name
HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)
Host
E Coli
Form/Format
Supplied as a liquid in PBS,pH 8.0, 1% BSA, 0.05% sodium azide. Conjugated to Biotin.
Purity/Purification
Purified by ion affinity and reverse phase HPLC to ? 99% as determined by SDS-PAGE and HPLC.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
May be stored at 4°C . For long term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Coupling Ratio
~5-10 moles of biotin per mole tat.Biological Activity||Biotin tat binds to solid phase murine anti-tat mAb as determined by capture ELISA using strepavidin-HRP as a secondary reagent.

Product Categories/Family for HIV-1 tat, Strain IIIB active protein

Molecular Biology; MB-Infectious Disease
Applications Tested/Suitable for HIV-1 tat, Strain IIIB active protein
Suitable for use in T Cell Research, ELISA and Western Blot.
Other applications not been tested.
Application Notes for HIV-1 tat, Strain IIIB active protein
A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA may be performed in 1:1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. tat/anti-tat mAb capture ELISA may be performed in 100ng mAb in solid phase and 1-10ng of biotin-tat.

Other Notes

Small volumes of HIV-1 tat, Strain IIIB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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