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RAP, Rat (RAPGEF3) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636670

RAP binds with high affinity to certain members of the low-density lipoprotein (LDL) receptor family such as LDL receptor-related protein (LRP), gp330/megalin, and the very low-density lipoprotein (VLDL) receptor and, once bound to these receptors,… More details

$900 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAP, Rat (Rapgef3), Recombinant Protein
Full Product Name
RAP, Recombinant, Rat (Receptor Associated Protein)
Product Gene Name
Rapgef3 recombinant protein
Host
E Coli
Form/Format
Supplied as a lyophilized powder from TBS, pH 7.5, 0.1% BSA, 0.09% sodium azide. Reconstitute with 100ul ddH2O.
Purity/Purification
Purified
>95% pure (SDS-PAGE)
Concentration
~1mg/ml (after reconstitution) (lot specific)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short term only. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -70 degree C. Reconstituted product is stable for 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
40kD
Chromosome Location
Chromosome: 7; NC_005106.3 (139423979..139445473, complement). Location: 7q36
3D Structure
ModBase 3D Structure for Q9Z1C8
Rat his:RAP
c-myc fusion partner.

Product Categories/Family for Rapgef3 recombinant protein

Molecular Biology; MB-Receptors
Applications Tested/Suitable for Rapgef3 recombinant protein
Western Blot (WB)
Application Notes for Rapgef3 recombinant protein
Suitable for use as protein standard in 1D and 2D SDS gel electrophoresis, immunoblotting and receptor-binding studies. Other applications not tested.
Optimal dilutions to be determined by the researcher.

Other Notes

Small volumes of Rapgef3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rapgef3 purchase

MBS636670 is a recombinant protein targeting Rapgef3. Also known as RAP, Recombinant, Rat (Receptor Associated Protein). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Rapgef3. It may not necessarily be applicable to this product.

NCBI GI #
11067419
NCBI GeneID
59326
NCBI Accession #
NP_067722.1
UniProt Primary Accession #
Q9Z1C8
UniProt Related Accession #
Q9Z1C8
GenBank Nucleotide #
NM_021690.1
NCBI Official Full Name
rap guanine nucleotide exchange factor 3
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 3
NCBI Official Symbol
Rapgef3
NCBI Official Synonym Symbols
Epac
UniProt Protein Name
Rap guanine nucleotide exchange factor 3
UniProt Synonym Protein Names
Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; cAMP-regulated guanine nucleotide exchange factor I
Protein Family
Rap guanine nucleotide exchange factor
UniProt Gene Name
Rapgef3
UniProt Synonym Gene Names
Epac; Epac1; EPAC 1; cAMP-GEFI
UniProt Entry Name
RPGF3_RAT
NCBI Protein Information
rap guanine nucleotide exchange factor 3; epac 1; cAMP-GEFI; exchange factor directly activated by cAMP 1; exchange protein directly activated by cAMP 1; cAMP-regulated guanine nucleotide exchange factor I (cAMP-GEFI)

NCBI Summary for Rapgef3

binds cAMP and activates the Ras superfamily guanine nucleotide binding protein (Rap1A)in a PKA-independent manner [RGD, Feb 2006]

UniProt Comments for Rapgef3

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.

Cellular Component: membrane; cytoplasm; cAMP-dependent protein kinase complex

Molecular Function: Rap guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; positive regulation of protein export from nucleus; positive regulation of angiogenesis; positive regulation of stress fiber formation; angiogenesis; regulation of protein kinase activity; positive regulation of peptidyl-serine phosphorylation; Rap protein signal transduction; positive regulation of GTPase activity
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Research Articles on Rapgef3

1. The cAMP-binding protein Epac critically modulates cardiac action potential duration by decreasing steady state potassium currents.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Rapgef3 recombinant protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Integration Of Energy Metabolism Pathway antibodies  Integration Of Energy Metabolism Pathway Diagram
 Integrin AlphaIIb Beta3 Signaling Pathway antibodies  Integrin AlphaIIb Beta3 Signaling Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Long-term Potentiation Pathway antibodies  Long-term Potentiation Pathway Diagram
 Long-term Potentiation Pathway antibodies  Long-term Potentiation Pathway Diagram

Diseases associated with Rapgef3 recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >26 publications with Rapgef3 and Neoplasms
 Inflammation Antibodies  >13 publications with Rapgef3 and Inflammation
 Hypertrophy Antibodies  >10 publications with Rapgef3 and Hypertrophy
 Heart Diseases Antibodies  >8 publications with Rapgef3 and Heart Diseases
 Carcinoma Antibodies  >7 publications with Rapgef3 and Carcinoma
 Edema Antibodies  >5 publications with Rapgef3 and Edema
 Necrosis Antibodies  >5 publications with Rapgef3 and Necrosis
 Pancreatic Neoplasms Antibodies  >2 publications with Rapgef3 and Pancreatic Neoplasms
 Kidney Diseases Antibodies  >2 publications with Rapgef3 and Kidney Diseases
 Pulmonary Disease, Chronic Obstructive Antibodies  >2 publications with Rapgef3 and Pulmonary Disease, Chronic Obstructive

Organs/Tissues associated with Rapgef3 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >23 publications with Rapgef3 and Heart
 Brain Antibodies  >12 publications with Rapgef3 and Brain
 Kidney Antibodies  >9 publications with Rapgef3 and Kidney
 Ovary Antibodies  >4 publications with Rapgef3 and Ovary
 Prostate Antibodies  >4 publications with Rapgef3 and Prostate
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