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MDM2, Ck1d, Active, GST-Tag (MDM2) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636694

MDM2 is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumour suppressor protein and notably MDM2 itself. The suppression of p53 activity by an amplified Mdm2 gene has been observed in many tumours, including… More details

$1335 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MDM2, CK1d, Active, GST-Tag, Recombinant Protein
Full Product Name
MDM2, CK1d, Active, Recombinant, Human, GST-Tag (Ubiquitin-protein Ligase E3 Mdm2, p53-binding Protein Mdm2, Oncoprotein Mdm2, Double Minute 2 Protein, Hdm2)
Form/Format
Supplied as a liquid in 50mM Tris/HCl pH7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Purity/Purification
Purified
~78% (SDS-PAGE, Coomassie blue staining)
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
~82kD, ~61kD

Testing Data of MDM2, CK1d, Active, GST-Tag recombinant protein

Activity was measured by titrating 164ng of MDM2/CK1δ enzyme in a ubiquitination assay.
MDM2 Recombinant Protein – Cat# MBS636694

SDS-Page of MDM2, CK1d, Active, GST-Tag recombinant protein

SDS-PAGE and Coomassie Stain: Purity was assessed by SDS- PAGE and Coomassie blue staining using 3ug of active MDM2/CK1d.
MDM2 Recombinant Protein, SDS-Page – Cat# MBS636694

Other Notes

Small volumes of MDM2, CK1d, Active, GST-Tag recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MDM2, CK1d, Active, GST-Tag purchase

MBS636694 is a recombinant protein targeting MDM2, CK1d, Active, GST-Tag. Also known as MDM2, CK1d, Active, Recombinant, Human, GST-Tag (Ubiquitin-protein Ligase E3 Mdm2, p53-binding Protein Mdm2, Oncoprotein Mdm2, Double Minute 2 Protein, Hdm2).
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