ASF1A Recombinant Protein – Cat# MBS636856

15% SDS-PAGE (3ug)

ASF1A, Aa1-204 (ASF1A) Recombinant Protein

Name: ASF1A Recombinant Protein; ASF1A, Aa1-204; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS636856
Price: 755 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636856

ASF1 anti-silencing function 1 homolog A, also known as ASF1A, belongs to H3/H4 family of histone chaperone proteins. ASF1A protein interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.… More details

$755 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ASF1A, aa1-204, Recombinant Protein

Full Product Name

ASF1A, aa1-204, Recombinant, Human (Anti-silencing Function 1A, ASF1 Anti-silencing Function 1 Homolog A, CCG1-interacting Factor A, CIA, CGI-98, DKFZp547E2110, hAsf1, hAsf1a, hCIA, Histone Chaperone ASF1A, HSPC146, RP3-329L24.1)

Product Gene Name

ASF1A recombinant protein

Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAKV QVNNVVVLDN PSPFYNPFQF EITFECIEDL SEDLEWKIIY VGSAESEEYD QVLDSVLVGP VPAGRHMFVF QADAPNPGLI PDADAVGVTV VLITCTYRGQ EFIRVGYYVN NEYTETELRE NPPVKPDFSK LQRNILASNP RVTRFHINWE DNTEKLEDAE SSNPNLQSLL STDALPSASK GWSTSENSLN VMLESHMDCM

Form/Format

Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, 1mM DTT, 10% glycerol.

Purity/Purification

Highly Purified
90% by SDS-PAGE

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

27kD

OMIM

609189

3D Structure

ModBase 3D Structure for Q9Y294

Endotoxin Level

1.0 EU /1ug of protein (LAL).

Product Categories/Family for ASF1A recombinant protein

Molecular Biology; MB-Chromatin Proteins

Other Notes

Small volumes of ASF1A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ASF1A purchase

MBS636856 is a recombinant protein targeting ASF1A. Also known as ASF1A, aa1-204, Recombinant, Human (Anti-silencing Function 1A, ASF1 Anti-silencing Function 1 Homolog A, CCG1-interacting Factor A, CIA, CGI-98, DKFZp547E2110, hAsf1, hAsf1a, hCIA, Histone Chaperone ASF1A, HSPC146, RP3-329L24.1).

Additional Details

NCBI/Uniprot data below describe general gene information for ASF1A. It may not necessarily be applicable to this product.

NCBI GI #

48146811

NCBI Accession #

CAG33628.1

UniProt Primary Accession #

Q9Y294

UniProt Secondary Accession #

Q6IA08; Q9P014

NCBI Official Full Name

ASF1A

UniProt Protein Name

Histone chaperone ASF1A

UniProt Synonym Protein Names

Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A

Protein Family

Histone chaperone

UniProt Gene Name

ASF1A

UniProt Synonym Gene Names

hAsf1; hAsf1a; CIA; hCIA

UniProt Entry Name

ASF1A_HUMAN

NCBI Summary for ASF1A

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008]

UniProt Comments for ASF1A

ASF1A: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Ubiquitously expressed. Belongs to the ASF1 family.

Protein type: Histone-binding; DNA repair, damage

Chromosomal Location of Human Ortholog: 6q22.31

Cellular Component: nucleoplasm; protein complex; nuclear chromatin; nucleus

Molecular Function: protein binding; histone binding; chromatin binding

Biological Process: osteoblast differentiation; nucleosome assembly; muscle cell differentiation; negative regulation of chromatin silencing; transcription, DNA-dependent; DNA replication-independent nucleosome assembly; chromatin modification; DNA repair; DNA replication-dependent nucleosome assembly

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ASF1A recombinant protein

Reference Product	PubMed Publications
HIRA recombinant protein	>21 publications with ASF1A and HIRA
TLK1 recombinant protein	>6 publications with ASF1A and TLK1
MCM2 recombinant protein	>2 publications with ASF1A and MCM2
TLK2 recombinant protein	>2 publications with ASF1A and TLK2
CHAF1B recombinant protein	>1 publications with ASF1A and CHAF1B
TAF1 recombinant protein	>1 publications with ASF1A and TAF1

Diseases associated with ASF1A recombinant protein

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>1 publications with ASF1A and Immune System Diseases
Anemia, Hemolytic Antibodies	>1 publications with ASF1A and Anemia, Hemolytic
Breast Neoplasms Antibodies	>1 publications with ASF1A and Breast Neoplasms
Liver Neoplasms Antibodies	>1 publications with ASF1A and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with ASF1A and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>1 publications with ASF1A and Liver Diseases
Necrosis Antibodies	>1 publications with ASF1A and Necrosis

Organs/Tissues associated with ASF1A recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3 publications with ASF1A and Blood
Muscle Antibodies	>2 publications with ASF1A and Muscle
Bone Antibodies	>1 publications with ASF1A and Bone
Bone Marrow Antibodies	>1 publications with ASF1A and Bone Marrow
Liver Antibodies	>1 publications with ASF1A and Liver
Testis Antibodies	>1 publications with ASF1A and Testis