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Protein Kinase A, Holoenzyme Type 1a Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS636992

Inactive holoenzyme consisting of one dimeric regulatory subunit type I alpha and two monomeric catalytic subunits (cAMP-free). Recombinant Human Protein Kinase A is purified by proprietary chromatographic techniques. More details

$750 / 0.001 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Protein Kinase A, Holoenzyme Type 1a, Active Protein
Full Product Name
Protein Kinase A, Holoenzyme Type 1a, Recombinant (PKA)
Host
E Coli
Form/Format
Supplied as a liquid in 50% glycerol.
Purity/Purification
Highly Purified
95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Recombinant Holo enzyme can be activated by adding the second messenger cAMP (Activation constant about 100nM) releasing two monomeric catalytic subunits.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.

Product Categories/Family for Protein Kinase A, Holoenzyme Type 1a active protein

Molecular Biology; MB-Protein Kinases
Application Notes for Protein Kinase A, Holoenzyme Type 1a active protein
The product is suitable for analysing PKA type I agonists (cAMP analogs) or antagonists.

Other Notes

Small volumes of Protein Kinase A, Holoenzyme Type 1a active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Protein Kinase A, Holoenzyme Type 1a purchase

MBS636992 is an active protein targeting Protein Kinase A, Holoenzyme Type 1a. Also known as Protein Kinase A, Holoenzyme Type 1a, Recombinant (PKA). Host: E Coli.
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