Frizzled 8, Fc Chimera Active Protein

Frizzled-8 is one of at least ten seven- transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor- related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes b-catenin and promotes gene transcription that is important in development and tissue maintenance. Human Frizzled-8 cDNA encodes 694aa including a 27aa signal peptide, a 247aa extracellular domain (ECD), the 7TM region (aa276-605), and a C-terminal cytoplasmic domain with a PDZ binding motif (aa606-694). The ECD includes a cysteine-rich region (CRD, aa30-151) that binds Wnts and is highly conserved among Frizzleds, and a linker region. Within aa28-172, human Frizzled-8 shares 99%, 99%, 90% and 85% aa identity with mouse, rat, Xenopus and zebrafish Frizzled-8, respectively. It also shares 82% aa identity with human Frizzled-5. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD. During mouse development, Frizzled-8 is expressed in tissues that are important for organizing the anterior-posterior axis. Interactions of Frizzled-8 with several non-Wnt ligands have been identified. Interaction with Frizzled-8 and LRP-6 allows R-spondins to activate b-catenin signaling pathways, while interaction with IGFBP-4 (insulin-like growth factor binding protein or CTGF (connective tissue growth factor) inhibits Wnt signaling. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.

Small volumes of Frizzled 8, Fc Chimera active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS637080 is an active protein targeting Frizzled 8, Fc Chimera. Also known as Frizzled 8, Fc Chimera, Recombinant, Human (FZD8, Frizzled-8, Fz-8, hFz8).