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SPHK2, Long Form (SPHK2) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637100

Human Sphingosine kinase 2 (GenBank Accession No.NM_020126), full length with N-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system. More details

$1110 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPHK2, Long Form, Active Protein
Full Product Name
SPHK2, Long Form, Recombinant, Human (Sphingosine kinase 2, SK 2)
Host
Baculovirus infected Sf9 cells
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol and 3mM DTT.
Purity/Purification
Purified
80% by SDS-PAGE
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight
74kD
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min.
Assay Buffer
50mM HEPES, pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 4uM D-erythrosphingosine, and 0.75ug/ml sphingosine kinase 2.

Product Categories/Family for SPHK2, Long Form active protein

Molecular Biology; MB-Protein Kinases
Application Notes for SPHK2, Long Form active protein
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Other Notes

Small volumes of SPHK2, Long Form active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPHK2, Long Form purchase

MBS637100 is an active protein targeting SPHK2, Long Form. Also known as SPHK2, Long Form, Recombinant, Human (Sphingosine kinase 2, SK 2). Host: Baculovirus infected Sf9 cells.
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