PPP1R1B Recombinant Protein – Cat# MBS637108

DARPP 32, His-Tag (PPP1R1B) Recombinant Protein

Name: PPP1R1B Recombinant Protein; DARPP 32, His-Tag; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS637108
Price: 865 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637108

DARPP-32 (dopamine and cyclic AMP regulated phosphoprotein, relative MR 32kD) is a cytosolic protein highly enriched in mediumsized spiny neurons of the neostriatum (1). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of… More details

$865 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DARPP 32, His-Tag (Ppp1r1b), Recombinant Protein

Full Product Name

DARPP 32, His-Tag, Recombinant, Human (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)

Product Gene Name

Ppp1r1b recombinant protein

Form/Format

Supplied as a liquid in Tris-buffered saline, 40% glycerol.

Purity/Purification

Purified
~90% by SDS-PAGE. The Recombinant DARPP-32 His fusion protein preparation shows two bands of 38kD and 26kD, the latter representing a form lacking ~10-12kD from the carboxy terminus. Both bands are excellent substrates for phosphorylation.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

21,781 Da

Chromosome Location

Chromosome: 11; NC_000077.6 (98348738..98357795). Location: 11 D; 11

3D Structure

ModBase 3D Structure for Q60829

Product Categories/Family for Ppp1r1b recombinant protein

Molecular Biology; MB-Enzymes; Phosphatase

Application Notes for Ppp1r1b recombinant protein

Suitable for use in Phosphatase assays.,

Other Notes

Small volumes of Ppp1r1b recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ppp1r1b purchase

MBS637108 is a recombinant protein targeting Ppp1r1b. Also known as DARPP 32, His-Tag, Recombinant, Human (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B).

Additional Details

NCBI/Uniprot data below describe general gene information for Ppp1r1b. It may not necessarily be applicable to this product.

NCBI GI #

1244402

NCBI GeneID

19049

NCBI Accession #

AAA93223.1

UniProt Primary Accession #

Q60829

UniProt Secondary Accession #

Q3URF2; Q6DV86; Q91XB5; A2A564

UniProt Related Accession #

Q60829

NCBI Official Full Name

DARPP-32, partial

NCBI Official Synonym Full Names

protein phosphatase 1, regulatory (inhibitor) subunit 1B

NCBI Official Symbol

Ppp1r1b

NCBI Official Synonym Symbols

Darpp32; AU040756; DARPP-32

UniProt Protein Name

Protein phosphatase 1 regulatory subunit 1B

UniProt Synonym Protein Names

DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

Protein Family

Protein phosphatase 1 regulatory

UniProt Gene Name

Ppp1r1b

UniProt Entry Name

PPR1B_MOUSE

NCBI Protein Information

protein phosphatase 1 regulatory subunit 1B; dopamine- and cAMP-regulated neuronal phosphoprotein

UniProt Comments for Ppp1r1b

DARPP-32: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Cellular Component: cell soma; cytoplasm; intracellular; nucleus

Molecular Function: D4 dopamine receptor binding; protein phosphatase inhibitor activity; type 1 serine/threonine specific protein phosphatase inhibitor activity; D1 dopamine receptor binding; D3 dopamine receptor binding; D5 dopamine receptor binding; D2 dopamine receptor binding

Biological Process: negative regulation of female receptivity; transcription, DNA-dependent; response to amphetamine; negative regulation of catalytic activity; visual learning; negative regulation of protein kinase activity; signal transduction

Research Articles on Ppp1r1b

1. DARPP32 has a role in pluripotent stem cell differentiation toward medium-sized spiny neurons

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Ppp1r1b recombinant protein

Products by Pathway	Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram
Cocaine Addiction Pathway antibodies	Cocaine Addiction Pathway Diagram
DARPP-32 Events Pathway antibodies	DARPP-32 Events Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram
Dopaminergic Synapse Pathway antibodies	Dopaminergic Synapse Pathway Diagram
Opioid Signalling Pathway antibodies	Opioid Signalling Pathway Diagram

Diseases associated with Ppp1r1b recombinant protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>51 publications with Ppp1r1b and Nervous System Diseases
Brain Diseases Antibodies	>39 publications with Ppp1r1b and Brain Diseases
Movement Disorders Antibodies	>39 publications with Ppp1r1b and Movement Disorders
Dyskinesias Antibodies	>29 publications with Ppp1r1b and Dyskinesias
Disease Models, Animal Antibodies	>28 publications with Ppp1r1b and Disease Models, Animal
Substance-Related Disorders Antibodies	>21 publications with Ppp1r1b and Substance-Related Disorders
Cognition Disorders Antibodies	>21 publications with Ppp1r1b and Cognition Disorders
Dyskinesia, Drug-Induced Antibodies	>10 publications with Ppp1r1b and Dyskinesia, Drug-Induced
Parkinsonian Disorders Antibodies	>10 publications with Ppp1r1b and Parkinsonian Disorders
Neurotoxicity Syndromes Antibodies	>10 publications with Ppp1r1b and Neurotoxicity Syndromes

Organs/Tissues associated with Ppp1r1b recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>145 publications with Ppp1r1b and Brain
Stomach Antibodies	>10 publications with Ppp1r1b and Stomach
Embryonic Tissue Antibodies	>4 publications with Ppp1r1b and Embryonic Tissue
Eye Antibodies	>3 publications with Ppp1r1b and Eye
Thyroid Antibodies	>2 publications with Ppp1r1b and Thyroid
Kidney Antibodies	>1 publications with Ppp1r1b and Kidney
Heart Antibodies	>1 publications with Ppp1r1b and Heart
Liver Antibodies	>1 publications with Ppp1r1b and Liver
Lung Antibodies	>1 publications with Ppp1r1b and Lung
Salivary Gland Antibodies	>1 publications with Ppp1r1b and Salivary Gland