Assay Conditions: Paraoxonase-1 catalyses the cleavage of phenyl acetate (4 mM) in 20mM Tris HCl buffer pH 8, containing 1mM CaCl2 resulting in phenol formation. The rate of formation of phenol is measured by monitoring the increase in absorbance at 270nm at 25ºC. One unit of arylesterase activity is equal to 1uM of phenol formed per minute. The activity is expressed in kU/L, based on the extinction coefficient of phenol of 1310 M-1 cm at 270nm, pH 8, and 25ºC.

Paraoxonase 1 Active Protein

Name: Active Protein; Paraoxonase 1; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS637128
Price: 650 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637128

Recombinant corresponding to human PON1, fused to 6X His-tag at C-terminal, expressed in CHO-K1 cells.Paraoxonase-1 is involved in the detoxification of organophosphate insecticides such as parathion. Paraoxonase-1 may also confer protection against coronary… More details

$650 / 10 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Paraoxonase 1, Active Protein

Full Product Name

Paraoxonase 1, Recombinant, Human

Product Synonym Names

Serum Paraoxonase/Arylesterase 1; PON 1; Aromatic Esterase 1

Host

CHO-K1 Cells

Form/Format

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 75mM sodium chloride, 0.05% Triton, 50% glycerol

Purity/Purification

Purified (SDS-PAGE).

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months after receipt at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

42.9kD

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source Note

Recombinant corresponding to human PON1, fused to 6X His-tag at C-terminal, expressed in CHO-K1 cells.

Activity

24.73U/ml

Product Categories/Family for Paraoxonase 1 active protein

Molecular Biology; MB-Enzymes; Phosphatase

Product References and Citations for Paraoxonase 1 active protein

1. Mackness B et al. (1998), Human Serum Paraoxonase Gen. Pharmacol. 31 (3) 329-336.

Other Notes

Small volumes of Paraoxonase 1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Paraoxonase 1 purchase

MBS637128 is an active protein targeting Paraoxonase 1. Also known as Paraoxonase 1, Recombinant, Human. Host: CHO-K1 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for Paraoxonase 1. It may not necessarily be applicable to this product.

NCBI GI #

345894407

NCBI Accession #

AEO20077.1

NCBI Official Full Name

paraoxonase 1

NCBI Summary for Paraoxonase 1

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.