Rabbit Anti-BRF1 Polyclonal Antibody – Cat# MBS6371432

Western Blot analysis of BRF1 expression in transfected 293T cell line by BRF1 polyclonal antibody. Lane 1: BRF1 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.

BRF1 Antibody – Rabbit Polyclonal

Name: BRF1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6371432
Price: 1165 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6371432

$1165 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BRF1, Polyclonal Antibody

Full Product Name

BRF1 (BRF, GTF3B, TAF3B2, TAF3C, Transcription Factor IIIB 90kD Subunit, B-related Factor 1, TATA Box-binding Protein-associated Factor, RNA Polymerase III, Subunit 2, MGC105048) APC

Product Gene Name

anti-BRF1 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Specificity

Recognizes human BRF1.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

OMIM

604902

3D Structure

ModBase 3D Structure for Q92994

Immunogen

Full length human BRF1, aa1-473 (NP_663718.1).

Immunogen Sequence

MTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEEECDPPSYTAGQRKLRMKQLEQVLSKKLEEVEGEISSYQDAIEIELENSRPKAKGGLASLAKDGSTEDTASSLCGEEDTEDEELEAAASHLNKDLYRELLGGAPGSSEAAGSPEWGGRPPALGSLLDPLPTAASLGISDSIRECISSQSSDPKDASGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKEARIAKEKELGIYKEHKPKKSCKRREPIQASTAREAIEKMLEQKKISSKINYSVLRGLSSAGGGSPHREDAQPEHSASARKLSRRRTPASRSGADPVTSVGKRLRPLVSTQPAKKVATGEALLPSSPTLGAEPARPQAVLVESGPVSYHADEEADEEEPDEEDGEPCVSALQMMGSNDYGCDGDEDDGY

Conjugate

APC

Note

Preservative Free

Product Categories/Family for anti-BRF1 antibody

Antibodies; Transcription Factors

Applications Tested/Suitable for anti-BRF1 antibody

Western Blot (WB)

Application Notes for anti-BRF1 antibody

Applications are based on unconjugated antibody.

Other Notes

Small volumes of anti-BRF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRF1 purchase

MBS6371432 is an antibody targeting BRF1. Also known as BRF1 (BRF, GTF3B, TAF3B2, TAF3C, Transcription Factor IIIB 90kD Subunit, B-related Factor 1, TATA Box-binding Protein-associated Factor, RNA Polymerase III, Subunit 2, MGC105048) APC. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BRF1. It may not necessarily be applicable to this product.

NCBI GI #

22035558

NCBI GeneID

2972

NCBI Accession #

NP_663718.1

UniProt Primary Accession #

Q92994

UniProt Related Accession #

Q92994

GenBank Nucleotide #

NM_145685.1

NCBI Official Full Name

transcription factor IIIB 90 kDa subunit isoform 3

NCBI Official Synonym Full Names

BRF1 RNA polymerase III transcription initiation factor subunit

NCBI Official Symbol

BRF1

NCBI Official Synonym Symbols

BRF; CFDS; hBRF; BRF-1; GTF3B; TAF3C; TAF3B2; TF3B90; TAFIII90; TFIIIB90; HEL-S-76p

UniProt Protein Name

Transcription factor IIIB 90 kDa subunit

UniProt Synonym Protein Names

B-related factor 1; BRF-1; hBRF; TAF3B2; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2

Protein Family

Transcription factor

UniProt Gene Name

BRF1

UniProt Synonym Gene Names

BRF; GTF3B; TAF3B2; TAF3C; TFIIIB90; hTFIIIB90; BRF-1; hBRF

UniProt Entry Name

TF3B_HUMAN

NCBI Protein Information

transcription factor IIIB 90 kDa subunit

NCBI Summary for BRF1

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]

UniProt Comments for BRF1

GTF3B: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter. Belongs to the TFIIB family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 14q

Cellular Component: nucleoplasm; transcription factor TFIIIB complex

Molecular Function: zinc ion binding; TATA-binding protein binding

Biological Process: transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; tRNA transcription; regulation of transcription, DNA-dependent; gene expression; regulation of mRNA stability; rRNA transcription

Disease: Cerebellofaciodental Syndrome

Research Articles on BRF1

1. Recent studies have demonstrated that Brf1 is overexpressed in most ER+ (estrogen receptor positive) cases of breast cancer and the change in cellular levels of Brf1 reflects the therapeutic efficacy and prognosis of this disease. It suggests that Brf1 may be a potential diagnosis biomarker and a therapeutic target of alcohol-associated breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.