• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

RAF Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637418

The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and… More details

$1065 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAF, Active Protein
Full Product Name
RAF, Recombinant, Human (A Raf, A-raf-1, ARaf Proto-oncogene Serine/threonine Protein Kinase, ARAF1, ARAF, Oncogene PKS2, PKS, PKS2, Proto-oncogene Pks, RAFA1, Ras-binding Protein DA-Raf, v-Raf Mouse Sarcoma 3611 Viral Oncogene Homolog)
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.
Purity/Purification
Purified
0.8
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight
63.6kD
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
50nM aRAF yields 8% conversion of 1uM ATP using MEK1 (K97R) as substrate.

Reaction Buffer:
10mM HEPES (pH7.4), 5mM MgCl2, 10uM MnCl2, 1mM DTT, 3uM Naorthovanadate, 1uM ATP.

Product Categories/Family for RAF active protein

Application Notes for RAF active protein
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Testing Data of RAF active protein

specific activity
RAF Active Protein – Cat# MBS637418

SDS-Page of RAF active protein

10% SDS-PAGE Coomassie staining. |Lane 1: 2ug R0495-05A|Lane 2: MW marker|MW: 63kD |Purity: ≥62%
RAF Active Protein, SDS-Page – Cat# MBS637418

Other Notes

Small volumes of RAF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAF purchase

MBS637418 is an active protein targeting RAF. Also known as RAF, Recombinant, Human (A Raf, A-raf-1, ARaf Proto-oncogene Serine/threonine Protein Kinase, ARAF1, ARAF, Oncogene PKS2, PKS, PKS2, Proto-oncogene Pks, RAFA1, Ras-binding Protein DA-Raf, v-Raf Mouse Sarcoma 3611 Viral Oncogene Homolog).

Additional Details

NCBI/Uniprot data below describe general gene information for RAF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
RAF

UniProt Comments for RAF

Antagonizes transcriptional repression of recombinase FLP by REP1-REP2. May play a role in plasmid partitioning.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000