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HIV-1 Tat, Strain IIIB (HIV) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637441

Full length (2 Exons, 86 amino acids) Recombinant HIV-1 IIIB tat produced in the E. coli Expression System. More details

$1455 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIV-1 tat, Strain IIIB, Active Protein
Full Product Name
HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1)
Host
E Coli
Form/Format
Supplied as a liquid in 0.2M KCl, 5mM glutathione. No stabilizers or preservatives added.
Purity/Purification
Affinity Purified
Purified by ion affinity and reverse phase HPLC to 99% as determined by SDS-PAGE and HPLC.
Specificity
This protein binds to murine monoclonal antibodies of defined epitope specificity and human serum polyclonal antibodies in ELISA and Western ELISA.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
The biological specificity of this protein was determined by LTR-CAT activation in scrapie-loaded HeLe Cells, and in U373 MG cells in the presence of 100uM chlorochine diphosphate. Tat concentrations in 1ug/ml range produced at least 25 fold increase in CAT activity. This protein inhibits DP4 (CD26) activity in vitro and DP4-dependent human T-cell activation in vivo.
Endotoxin Level
Less than 0.01EU/mg of protein as determined by BioWhittaker Kinetic QCL Kit.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for HIV-1 tat, Strain IIIB active protein

Molecular Biology; MB-Infectious Disease
Application Notes for HIV-1 tat, Strain IIIB active protein
A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute tat stock solution in saline-citrate buffer (150mM NaCl, 50mM sodium citrate, pH 6.5) immediately before use. Tat readily oxidizes in buffer solutions which may change its LTR dependent transcriptional activation activity. Transcriptional activation assays with tat are performed in 1-5ug/ml range. ELISA and Western ELISA require tat in 10-100ng protein range.

Other Notes

Small volumes of HIV-1 tat, Strain IIIB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIV-1 tat, Strain IIIB purchase

MBS637441 is an active protein targeting HIV-1 tat, Strain IIIB. Also known as HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1). Host: E Coli.
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