ENOPH1 Recombinant Protein – Cat# MBS637483

15% SDS-PAGE (3ug)

MASA, Aa1-261 (ENOPH1) Recombinant Protein

Name: ENOPH1 Recombinant Protein; MASA, Aa1-261; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS637483
Price: 755 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637483

Enolase-phosphatase E1, also known as ENOPH1, is a member of the MasA family of the HAD (halo-acid dehalogenase)-like hydrolase superfamily. ENOPH1 is a bifunctional enzyme, exhibiting both phosphatase and atypical enolase activities. ENOPH1 plays an… More details

$755 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MASA, aa1-261 (ENOPH1), Recombinant Protein

Full Product Name

MASA, aa1-261, Recombinant, Human (Enolase Phosohatase E1, ENOPH1, 2,3-diketo-5-methylthio-1-phosphopentane Phosphatase, DKFZp586M0524, FLJ12594, MST145, MSTP145)

Product Gene Name

ENOPH1 recombinant protein

Form/Format

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 100mM sodium chloride, 10% glycerol.

Purity/Purification

Highly Purified
~90% (SDS-PAGE)

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

~31kD

Chromosome Location

Chromosome: 4; NC_000004.11 (83351726..83382245). Location: 4q21.22

3D Structure

ModBase 3D Structure for Q9UHY7

Product Categories/Family for ENOPH1 recombinant protein

Molecular Biology; MB-Enzymes; Phosphatase

Other Notes

Small volumes of ENOPH1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENOPH1 purchase

MBS637483 is a recombinant protein targeting ENOPH1. Also known as MASA, aa1-261, Recombinant, Human (Enolase Phosohatase E1, ENOPH1, 2,3-diketo-5-methylthio-1-phosphopentane Phosphatase, DKFZp586M0524, FLJ12594, MST145, MSTP145).

Additional Details

NCBI/Uniprot data below describe general gene information for ENOPH1. It may not necessarily be applicable to this product.

NCBI GI #

48146399

NCBI GeneID

58478

NCBI Accession #

CAG33422.1

UniProt Primary Accession #

Q9UHY7

UniProt Secondary Accession #

Q7Z4C5; Q9BVC2

UniProt Related Accession #

Q9UHY7

NCBI Official Full Name

MASA

NCBI Official Synonym Full Names

enolase-phosphatase 1

NCBI Official Symbol

ENOPH1

NCBI Official Synonym Symbols

E1; MASA; mtnC; MST145

UniProt Protein Name

Enolase-phosphatase E1

UniProt Synonym Protein Names

2,3-diketo-5-methylthio-1-phosphopentane phosphatase; MASA homolog

UniProt Gene Name

ENOPH1

UniProt Synonym Gene Names

MASA

UniProt Entry Name

ENOPH_HUMAN

NCBI Protein Information

enolase-phosphatase E1; MASA homolog; acireductone synthase; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase

UniProt Comments for ENOPH1

ENOPH1: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK- MTPene). Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.77; Phosphatase (non-protein); Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 4q21.22

Cellular Component: cytosol; nucleus

Molecular Function: acireductone synthase activity; magnesium ion binding

Biological Process: dephosphorylation; methionine salvage; sulfur amino acid metabolic process; polyamine metabolic process

Research Articles on ENOPH1

1. the crystal structure of MASA and its complex with a substrate analog

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ENOPH1 recombinant protein

Products by Pathway	Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Metabolism Of Polyamines Pathway antibodies	Metabolism Of Polyamines Pathway Diagram
Methionine Salvage Pathway antibodies	Methionine Salvage Pathway Diagram
Methionine Salvage Pathway antibodies	Methionine Salvage Pathway Diagram
Methionine Salvage Pathway antibodies	Methionine Salvage Pathway Diagram
S-methyl-5-thio-alpha-D-ribose 1-phosphate Degradation I Pathway antibodies	S-methyl-5-thio-alpha-D-ribose 1-phosphate Degradation I Pathway Diagram
Sulfur Amino Acid Metabolism Pathway antibodies	Sulfur Amino Acid Metabolism Pathway Diagram

Diseases associated with ENOPH1 recombinant protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>1 publications with ENOPH1 and Nervous System Diseases
Congenital Abnormalities Antibodies	>1 publications with ENOPH1 and Congenital Abnormalities
Abnormalities, Multiple Antibodies	>1 publications with ENOPH1 and Abnormalities, Multiple

Organs/Tissues associated with ENOPH1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>1 publications with ENOPH1 and Brain