Restrictin (TNR) Active Protein

Name: TNR Active Protein; Restrictin; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS637509
Price: 945 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637509

TenascinR (TNR) is an extracellular matrix glycoprotein belonging to the tenascin family of adhesion proteins. 1-3 TNR is expressed in the central nervous system by oligodendrocytes and selected inhibitory interneurons. It shows highest expression during the… More details

$945 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Restrictin (TNR), Active Protein

Full Product Name

Restrictin, Recombinant, Human (Janusin, Tenascin R)

Product Gene Name

TNR active protein

Form/Format

Supplied as a lyophilized powder in PBS.

Purity/Purification

Highly Purified
90%, as determined by SDS-PAGE and visualized by silver stain.

Preparation and Storage

Lyophilized samples are stable for up to twelve months from date of receipt at -20 degree C. Upon reconstitution, this protein can be stored under sterile conditions at 2 degree -8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Molecular Weight

Based on N-terminal sequencing, the recombinant human TenascinR starts at Glu 34 and has a calculated molecular mass of approximately 146.8 kD. As a result of glycosylation, the recombinant human TenascinR migrates as an approximately 157-180 kD protein i

Chromosome Location

Chromosome: 8; NC_006095.3 (6987423..7036376). Location: chromosome: 8

3D Structure

ModBase 3D Structure for Q00546

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Activity

Measured by its ability to bind rhContactin-1/Fc in a functional ELISA. rhTenascin-R, immobilized at 5ug/ml (100 L/well), will bind rhContactin-1/Fc with an apparent KD < 10 nM.

Endotoxin Level

< 1.0 EU per 1 g of the protein as determined by the LAL method.

Reconstitution

It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.

Product Categories/Family for TNR active protein

Molecular Biology; MB-Extracellular Matrix Proteins

Other Notes

Small volumes of TNR active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNR purchase

MBS637509 is an active protein targeting TNR. Also known as Restrictin, Recombinant, Human (Janusin, Tenascin R).

Additional Details

NCBI/Uniprot data below describe general gene information for TNR. It may not necessarily be applicable to this product.

NCBI GI #

63614

NCBI GeneID

396213

NCBI Accession #

CAA45920.1

UniProt Primary Accession #

Q00546

UniProt Related Accession #

Q00546

NCBI Official Full Name

restrictin-precursor

NCBI Official Synonym Full Names

tenascin R (restrictin, janusin)

NCBI Official Symbol

TNR

UniProt Protein Name

Tenascin-R

UniProt Synonym Protein Names

Restrictin

Protein Family

Tenascin

UniProt Gene Name

TNR

UniProt Synonym Gene Names

TN-R

UniProt Entry Name

TENR_CHICK

NCBI Protein Information

tenascin-R; TN-R; restrictin

UniProt Comments for TNR

Function: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. Involved in cell attachment and neurite formation. Interaction with CNTN1 enhances the neurite outgrowth. Ref.2

Subunit structure: Forms homodimers and homotrimers. Interacts with CNTN1, NFASC and CSPG5. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5

Subcellular location: Secreted › extracellular space › extracellular matrix. Note: May be attach to the cell surface of neural cells.

Tissue specificity: Brain specific. Ref.1

Developmental stage: Expression weakly detectable at E6 embryo, reaches a maximum at E16 and declines in the adult. Ref.1

Domain: The N-terminus cysteine-rich segment may mediate the formation of oligomers. The fibronectin type-III 2-3 mediate the binding to contactin 1. The fibronectin type-III 9 mediates the cell attachment. The fibronectin type-III 2-5 mediate NFASC binding. The fibrinogen C-terminal domain mediates interaction with CSPG5. Ref.5

Sequence similarities: Belongs to the tenascin family.Contains 4 EGF-like domains.Contains 1 fibrinogen C-terminal domain.Contains 9 fibronectin type-III domains.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with TNR active protein

Products by Pathway	Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram

Diseases associated with TNR active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>41 publications with TNR and Nervous System Diseases
Neurodegenerative Diseases Antibodies	>29 publications with TNR and Neurodegenerative Diseases
Mental Disorders Antibodies	>22 publications with TNR and Mental Disorders
Disease Models, Animal Antibodies	>9 publications with TNR and Disease Models, Animal
Dementia Antibodies	>9 publications with TNR and Dementia
Cognition Disorders Antibodies	>8 publications with TNR and Cognition Disorders
Movement Disorders Antibodies	>8 publications with TNR and Movement Disorders
Death Antibodies	>4 publications with TNR and Death
Inflammation Antibodies	>2 publications with TNR and Inflammation
Weight Loss Antibodies	>2 publications with TNR and Weight Loss

Organs/Tissues associated with TNR active protein

Organ/Tissue Name	Pubmed Publications
Head Antibodies	>9 publications with TNR and Head