Inducible Nitric Oxide Synthase, Rat, Western Blot Control (NOS2) Recombinant Protein

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline

Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; vesicle membrane; nucleus; cytosol

Molecular Function: protein homodimerization activity; FAD binding; nitric-oxide synthase activity; beta-catenin binding; cAMP-dependent protein kinase regulator activity; Hsp90 protein binding; actin binding; protein kinase binding; calmodulin binding; amino acid binding; NADPH-hemoprotein reductase activity; FMN binding; cadherin binding; iron ion binding; heme binding; nitric-oxide synthase binding; NADP binding; receptor binding

Biological Process: retinoic acid receptor signaling pathway; wound healing; cGMP-mediated signaling; positive regulation of apoptosis; response to hormone stimulus; response to lipopolysaccharide; positive regulation of killing of cells of another organism; positive regulation of vasodilation; defense response to Gram-negative bacterium; signal transduction; regulation of heart contraction; response to estradiol stimulus; regulation of blood pressure; negative regulation of blood pressure; inflammatory response; nitric oxide biosynthetic process; glycoside metabolic process; aging; superoxide metabolic process; positive regulation of guanylate cyclase activity; ovulation from ovarian follicle; regulation of cell proliferation; response to mechanical stimulus; endothelial cell proliferation; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; blood vessel remodeling; G-protein signaling, coupled to cGMP nucleotide second messenger; response to activity; regulation of protein kinase activity; negative regulation of protein catabolic process; regulation of insulin secretion; nitric oxide mediated signal transduction