TMPRSS15 Active Protein – Cat# MBS637611

Fig.1. Each reaction contains 50ug of purified IL-8 fusion protein and varying amount of EK. The reactions were incubated at 22°C for 16h and analyzed on Coomassie stained SDS-PAGE (15%). Units of enzyme used per reaction is listed as follows:

Enterokinase, Light Chain, Porcine (TMPRSS15) Active Protein

Name: TMPRSS15 Active Protein; MyBioSource
Brand: MyBioSource
SKU: MBS637611
Price: 410 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637611

MBS637611 is an active protein targeting TMPRSS15. Also known as Enterokinase, Light Chain, Recombinant, Porcine (Enteropeptidase). More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Enterokinase, Light Chain, Porcine (TMPRSS15), Active Protein

Full Product Name

Enterokinase, Light Chain, Recombinant, Porcine (Enteropeptidase)

Product Gene Name

TMPRSS15 active protein

Form/Format

The enzyme component is supplied as a liquid in proprietary buffer formulation.

Purity/Purification

Highly Purified
90%. Enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases.

Preparation and Storage

Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. Note: Can stand at 37 degree C for seven days without losing activity. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

Theoretical MW: 21.88kD. Apparent MW on SDS-PAGE: ~40kD

Chromosome Location

Chromosome: 1; AC_000158.1 (18058709..18207251). Location: chromosome: 1

3D Structure

ModBase 3D Structure for P98072

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Immunogen

>= 98%

Activity

2U/ul.

Supplied With

1X Enterokinase Dilution/Storage Buffer, 1x2ml
10X Enterokinase Cleavage Buffer, 1x1ml
Cleavage Control Protein (lyophilized), 1x10ug. (The MW of control protein is 26kD before cleavage. After cleavage, the MWs of the two fragments are 17kD and 9kD).

Unit Definition

One unit is defined as the amount of enzyme needed to cleave 50ug of fusion protein in 16 hours to 95% completion at 22 degree C in a buffer containing 25 mM Tris-HCl, pH 8.0.

Product Categories/Family for TMPRSS15 active protein

Molecular Biology; MB-Enzymes

Other Notes

Small volumes of TMPRSS15 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TMPRSS15. It may not necessarily be applicable to this product.

NCBI GI #

27806097

NCBI GeneID

282009

NCBI Accession #

NP_776864.1

UniProt Primary Accession #

P98072

UniProt Related Accession #

P98072

GenBank Nucleotide #

NM_174439.2

NCBI Official Full Name

enteropeptidase

NCBI Official Symbol

TMPRSS15

NCBI Official Synonym Symbols

PRSS7

UniProt Protein Name

Enteropeptidase

UniProt Synonym Protein Names

Enterokinase; Serine protease 7; Transmembrane protease serine 15

Protein Family

Enteropeptidase

UniProt Gene Name

TMPRSS15

UniProt Synonym Gene Names

ENTK; PRSS7

UniProt Entry Name

ENTK_BOVIN

NCBI Protein Information

enteropeptidase; enterokinase; serine protease 7; transmembrane protease serine 15; protease, serine, 7 (enterokinase)

UniProt Comments for TMPRSS15

Function: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.

Catalytic activity: Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.

Subunit structure: Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond. Ref.4

Subcellular location: Membrane; Single-pass type II membrane protein

Probable.

Tissue specificity: Intestinal brush border.

Post-translational modification: The chains are derived from a single precursor that is cleaved by a trypsin-like protease.

Sequence similarities: Belongs to the peptidase S1 family.Contains 2 CUB domains.Contains 2 LDL-receptor class A domains.Contains 1 MAM domain.Contains 1 peptidase S1 domain.Contains 1 SEA domain.Contains 1 SRCR domain.

Research Articles on TMPRSS15

1. substrate specificities of porcine and bovine enteropeptidases were investigated using the peptide Val-(Asp)(4)-Lys-Ile-Val-Gly and its various analogs with mutations in the (Asp)(4)-Lys sequence as substrates

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.