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Atk1, Inactive, N-Terminal His Tag, Serine/Threonine Kinase Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637714

Human Akt1 (GenBank Accession No. NM_001014432), (a.a. 1-480) with N-terminal HIS6 tag, MW 60.2kD, expressed in an Baculovirus infected Sf9 cell expression system. More details

$1060 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Atk1, inactive, N-terminal His tag, Serine/Threonine Kinase, Active Protein
Full Product Name
Atk1, inactive, Recombinant, Human, N-terminal His tag, Serine/Threonine Kinase
Host
Baculovirus infected Sf9 cells
Species Reactivity
Human
Form/Format
25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.
Purity/Purification
80% by SDS-PAGE.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
100U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of Crosstide substrate per minute at pH 7.4 and 30 degree C.
Assay Buffer
50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP.
useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Other Notes

Small volumes of Atk1, inactive, N-terminal His tag, Serine/Threonine Kinase active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Atk1, inactive, N-terminal His tag, Serine/Threonine Kinase purchase

MBS637714 is an active protein targeting Atk1, inactive, N-terminal His tag, Serine/Threonine Kinase. Also known as Atk1, inactive, Recombinant, Human, N-terminal His tag, Serine/Threonine Kinase. Reactive in Human. Host: Baculovirus infected Sf9 cells.
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