• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DNA Polymerase I Klenow Fragment, Exo-Minus (DNA) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637734

Klenow fragment, exo, exhibits 5'-3' polymerase activity of DNA polymerase I, E. coli. It lacks 3'-5' and 5'-3' exonuclease activities. It is purified from an E. coli strain carrying a modified DNA polymerase I large fragment overproducing plasmid. Klenow… More details

$585 / 300 Units +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA Polymerase I Klenow Fragment, Exo-Minus, Recombinant Protein
Full Product Name
DNA Polymerase I Klenow Fragment, Exo-Minus, Recombinant
Host
E Coli
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 7.4, 0.1mM EDTA, 1mM DTT, 50% glycerol.
Purity/Purification
Molecular Biology Grade
Preparation and Storage
For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quality Control
Endodeoxyribonuclease Assay:
No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of 20u of Klenow Fragment, exo-. with 1ug of pBR322 DNA in 50ul of reaction buffer for 4 hours at 37 degree C.
Unit Definition
1u incorporates 10nm of total deoxynucleotides into a DE-81 absorbable form using poly(dA-dT)• poly(dA-dT) as a template-primer in 30 minutes at 37ºC

Product Categories/Family for DNA Polymerase I Klenow Fragment, Exo-Minus recombinant protein

Cloning; Cloning-Enzymes
Application Notes for DNA Polymerase I Klenow Fragment, Exo-Minus recombinant protein
Suitable for use in labeling of DNA by the random primer method; labeling recessed 3'-termini of double-stranded DNA; DNA sequencing by the method of Sanger, F., et al.; Strand Displacement Amplification (SDA).

Other Notes

Small volumes of DNA Polymerase I Klenow Fragment, Exo-Minus recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNA Polymerase I Klenow Fragment, Exo-Minus purchase

MBS637734 is a recombinant protein targeting DNA Polymerase I Klenow Fragment, Exo-Minus. Also known as DNA Polymerase I Klenow Fragment, Exo-Minus, Recombinant. Host: E Coli.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000