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HIV-1 Nef, Strain IIIB (HIV) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637848

MBS637848 is an active protein targeting HIV-1 nef, Strain IIIB. Also known as HIV-1 nef, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin). Recombiant full lenght HIV-1 nef, Strain IIIB expressed in E.coli. More details

$1000 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIV-1 nef, Strain IIIB, Active Protein
Full Product Name
HIV-1 nef, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)
Form/Format
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Purity/Purification
Purified by glutathione affinity chromatography and the purified protein is thrombin-cleaved to produce nef of ?95% purity, as determined by SDS-PAGE, reduced.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Coupling Ratio
~5-10 moles of biotin per mole nef.
Biological Activity
Binds to solid phase murine anti-nef monoclonal antibodies as determined by capture ELISA using strepavidin-HRP as a secondary reagent.

Product Categories/Family for HIV-1 nef, Strain IIIB active protein

Molecular Biology; MB-Infectious Disease
Applications Tested/Suitable for HIV-1 nef, Strain IIIB active protein
Suitable for use in T-Cell Research, ELISA and Western ELISA. Other applications not tested.
Application Notes for HIV-1 nef, Strain IIIB active protein
ELISA: 1:1000
Capture ELISA: 1-10ng

Optimal dilutions to be determined by the researcher.

Other Notes

Small volumes of HIV-1 nef, Strain IIIB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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