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RPA3 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637851

The 14kD subunit of human RPA was cloned from HeLa cell cDNA library. The RPA3 gene has a 692bp sequence with an open reading frame encoding a protein of 121 amino acids. The deduced amino acid sequence demonstrates limited similiarity to the small subunit of… More details

$615 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RPA3, Recombinant Protein
Full Product Name
RPA3 (Replication Protein A3, 14kD) Recombinant
Product Gene Name
RPA3 recombinant protein
Sequence
121aa, Full-lengt h RPA3
Form/Format
Supplied as a liquid in 10mM Tris,, pH 8.0, 0.1% triton X-100, 0.002% sodium azide, 10mM DTT.
Purity/Purification
Highly Purified
Highly purified (95%)
Preparation and Storage
For long-term storage, aliquot to avoid repeated freezing and thawing. Freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Molecular Weight
13,569 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (7676347..7758238, complement). Location: 7p22
OMIM
179837
3D Structure
ModBase 3D Structure for P35244
Tag Fusion Partner
His-tag at N-terminus
Quality Control
SDS-PAGE

Product Categories/Family for RPA3 recombinant protein

Molecular Biology; MB-Transcription Factors
Applications Tested/Suitable for RPA3 recombinant protein
ELISA (EL/EIA)
Application Notes for RPA3 recombinant protein
Suitable for use in ELISA, Western Blot and MS.

SDS-Page of RPA3 recombinant protein

SDS: Analysis of RPA3 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.
RPA3 Recombinant Protein – Cat# MBS637851

Other Notes

Small volumes of RPA3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPA3 purchase

MBS637851 is a recombinant protein targeting RPA3. Also known as RPA3 (Replication Protein A3, 14kD) Recombinant.

Additional Details

NCBI/Uniprot data below describe general gene information for RPA3. It may not necessarily be applicable to this product.

NCBI GI #
4506587
NCBI GeneID
6119
NCBI Accession #
NP_002938.1
UniProt Primary Accession #
P35244
UniProt Secondary Accession #
Q549U6
UniProt Related Accession #
P35244
GenBank Nucleotide #
NM_002947.3
NCBI Official Full Name
replication protein A 14 kDa subunit
NCBI Official Synonym Full Names
replication protein A3, 14kDa
NCBI Official Symbol
RPA3
NCBI Official Synonym Symbols
REPA3
UniProt Protein Name
Replication protein A 14 kDa subunit
UniProt Synonym Protein Names
Replication factor A protein 3
Protein Family
Replication protein
UniProt Gene Name
RPA3
UniProt Synonym Gene Names
REPA3; RPA14; RP-A p14
UniProt Entry Name
RFA3_HUMAN
NCBI Protein Information
replication protein A 14 kDa subunit; RP-A p14; RF-A protein 3; replication factor A protein 3

UniProt Comments for RPA3

RPA3: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: nucleoplasm; DNA replication factor A complex

Molecular Function: protein binding; damaged DNA binding; single-stranded DNA binding

Biological Process: mismatch repair; regulation of mitotic cell cycle; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; regulation of cell proliferation; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle
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Research Articles on RPA3

1. Data indicate taht candidate genes ACTB, BZW, OCM, MACC1, NXPH1, PRPS1L1, RAC1 and RPA3, which lie within the DFNB90 region, were sequenced and no potentially causal variants were identified.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RPA3 recombinant protein

 Reference Product  PubMed Publications
 RPA2 recombinant protein  >35 publications with RPA3 and RPA2
 RPA1 recombinant protein  >25 publications with RPA3 and RPA1
 RPA4 recombinant protein  >6 publications with RPA3 and RPA4
 PCNA recombinant protein  >3 publications with RPA3 and PCNA

Pathways associated with RPA3 recombinant protein

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway antibodies  Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with RPA3 recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with RPA3 and Neoplasms
 Neoplasm Metastasis Antibodies  >1 publications with RPA3 and Neoplasm Metastasis
 Weight Loss Antibodies  >1 publications with RPA3 and Weight Loss
 Precancerous Conditions Antibodies  >1 publications with RPA3 and Precancerous Conditions
 Kidney Diseases Antibodies  >1 publications with RPA3 and Kidney Diseases

Organs/Tissues associated with RPA3 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5 publications with RPA3 and Brain
 Blood Antibodies  >3 publications with RPA3 and Blood
 Muscle Antibodies  >2 publications with RPA3 and Muscle
 Heart Antibodies  >2 publications with RPA3 and Heart
 Lymph Antibodies  >1 publications with RPA3 and Lymph
 Skin Antibodies  >1 publications with RPA3 and Skin
 Lymph Node Antibodies  >1 publications with RPA3 and Lymph Node
 Kidney Antibodies  >1 publications with RPA3 and Kidney
 Lung Antibodies  >1 publications with RPA3 and Lung
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