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Growth Hormone, Pituitary, 20kD Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS637928

Recombinant Human GH-20K is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20322 Dalton. More details

$995 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Growth Hormone, Pituitary, 20kD, Active Protein
Full Product Name
Growth Hormone, Pituitary, Recombinant, Human, 20kD (hGH)
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Th r-Gly-Ser.
Host
E Coli
Form/Format
Supplied as a lyophilized powder in 0.0045mM NaHCO3, pH 10.
Purity/Purification
Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 0.4% NaHCO3 and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
rHuGH-20K is fully biologically active when compared to World Health Organization (WHO) reference standard.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of GH-20K.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.73 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH-20K as a Reference Standard.
Reconstitution
Reconstitute and dilute the lyophilized GH-20K in 0.4% NaHCO3 adjusted to pH 10, which can then be further diluted to other aqueous solutions. It is recommended when diluting to below 10ug/ml that the dilution solution will contain carrier protein (BSA or other) to avoid non-specific absorption of the hGH to plastic tubes.

Product Categories/Family for Growth Hormone, Pituitary, 20kD active protein

Molecular Biology; MB-Hormones; Steroids

Other Notes

Small volumes of Growth Hormone, Pituitary, 20kD active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Growth Hormone, Pituitary, 20kD purchase

MBS637928 is an active protein targeting Growth Hormone, Pituitary, 20kD. Also known as Growth Hormone, Pituitary, Recombinant, Human, 20kD (hGH). Host: E Coli.
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