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GRAP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6380230

Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. More details

$1165 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GRAP2, Polyclonal Antibody
Full Product Name
GRAP2 (GRB2 Related Adaptor Protein 2, GRAP-2, GADS, GRB2-like Protein, GRB2L, GRBLG, GrbX, Grf40 Adapter Protein, Grf-40, GRID, Growth Factor Receptor Binding Protein, GRPL, Mona, p38, SH3-SH2-SH3 Adapter Mona) (PE)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rabbit
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human GRAP2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
OMIM
604518
3D Structure
ModBase 3D Structure for O75791
Immunogen
Full length human GRAP2, aa1-330 (NP_004801.1).
Immunogen Sequence
MEAVAKFDFTASGEDELSFHTGDVLKILSNQEEWFKAELGSQEGYVPKNFIDIQFPKWFHEGLSRHQAENLLMGKEVGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDNKGNYFLWTEKFPSLNKLVDYYRTNSISRQKQIFLRDRTREDQGHRGNSLDRRSQGGPHLSGAVGEEIRPSMNRKLSDHPPTLPLQQHQHQPQPPQYAPAPQQLQQPPQQRYLQHHHFHQERRGGSLDINDGHCGTGLGSEMNAALMHRRHTDPVQLQAAGRVRWARALYDFEALEDDELGFHSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYVAPMTR
Conjugate
PE
Note
Preservative Free

Product Categories/Family for anti-GRAP2 antibody

Applications Tested/Suitable for anti-GRAP2 antibody
Western Blot (WB)
Application Notes for anti-GRAP2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GRAP2 antibody

GRAP2 rabbit polyclonal antibody. Western Blot analysis of GRAP2 expression in Jurkat.
Rabbit Anti-GRAP2 Polyclonal Antibody – Cat# MBS6380230

Western Blot (WB) of anti-GRAP2 antibody

Western Blot analysis of GRAP2 expression in transfected 293T cell line by GRAP2 polyclonal antibody. Lane 1: GRAP2 transfected lysate (37.9kD). Lane 2: Non-transfected lysate
Rabbit Anti-GRAP2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6380230

Testing Data of anti-GRAP2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between GRAP2 and CSF1R. HeLa cells were stained with GRAP2 rabbit purified polyclonal 1:1200 and CSF1R mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Rabbit Anti-GRAP2 Polyclonal Antibody, Testing Data – Cat# MBS6380230

Other Notes

Small volumes of anti-GRAP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GRAP2 purchase

MBS6380230 is an antibody targeting GRAP2. Also known as GRAP2 (GRB2 Related Adaptor Protein 2, GRAP-2, GADS, GRB2-like Protein, GRB2L, GRBLG, GrbX, Grf40 Adapter Protein, Grf-40, GRID, Growth Factor Receptor Binding Protein, GRPL, Mona, p38, SH3-SH2-SH3 Adapter Mona) (PE). Reactive in Human, Rabbit. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GRAP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens GRB2 related adaptor protein 2 (GRAP2), transcript variant 3, mRNA
NCBI Official Synonym Full Names
GRB2 related adaptor protein 2
NCBI Official Symbol
GRAP2
NCBI Official Synonym Symbols
P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
UniProt Protein Name
GRB2-related adapter protein 2
UniProt Synonym Protein Names
Adapter protein GRID; GRB-2-like protein; GRB2L; GRBLG; GRBX; Grf40 adapter protein; Grf-40; Growth factor receptor-binding protein; Hematopoietic cell-associated adapter protein GrpL; P38; Protein GADS; SH3-SH2-SH3 adapter Mona
UniProt Gene Name
GRAP2
UniProt Synonym Gene Names
GADS; GRB2L; GRID; GRB2L; Grf-40
UniProt Entry Name
GRAP2_HUMAN
NCBI Protein Information
GRB2-related adapter protein 2

NCBI Summary for GRAP2

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
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UniProt Comments for GRAP2

GADS: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. Belongs to the GRB2/sem-5/DRK family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol; endosome

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: positive regulation of signal transduction; cell-cell signaling; Ras protein signal transduction; T cell costimulation; innate immune response; T cell receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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