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Dll1:fc (DLL1) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638035

The Notch ligand delta-like protein 1 (DLL1) is essential for postnatal arteriogenesis and contributes to tumor progression. DLL1 is involved in differentiation and self-renewal of adipocyte stem cells. Blocks the differentiation of progenitor cells into the… More details

$540 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DLL1:Fc, Active Protein
Full Product Name
DLL1, Recombinant, Mouse:Fc (Delta-like Protein 1, Delta 1)
Sequence
Signal peptide and extracellu lar domain of mouse DLL1 (aa 1-545) is fused at the C-terminus to the Fc portion of human IgG1.
Host
Human, Mouse, HEK 293 cells
Form/Format
Supplied as a Lyophilized powder from PBS, Reconstitute with 50ul sterile ddH2O
Purity/Purification
Highly Purified
>90% (SDS-PAGE)
Specificity
Interacts with Notch receptors.
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
~100kD (SDS-PAGE)
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Inhibits adipogenesis of 3T3L-1 cells. Induces Hes-1 in 3T3L-1 cells.
Endotoxin
<0.1 EU/ug (LAL)

Product Categories/Family for DLL1:Fc active protein

Molecular Biology; MB-Notch Proteins

Testing Data of DLL1:Fc active protein

Adipogenesis inhibition of 3T3L1 cells: 3T3L1 cells were maintained in DMEM, w/10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1uM Dexamethasone, 0.5mM IBMX, 10ug/ml lnsulin (day 0) until day 2. The medium was replaced every 2 days w/new medium containing insulin in the presence or absence of 044747 (5ug/ml) and hCD137-Fc (Fc control). Staining w/Oil Red O was performed on day 7. Cells were washed twice w/PBS, fixed w/3.7% formalin, and stained w/0.5% filtered Oil Red O in propylene glycol.
DLL1 Active Protein – Cat# MBS638035

Western Blot (WB) of DLL1:Fc active protein

Induction of Hes-1 with the treatment of 044747: A mouse preadpipocyte cell line, 3T3L1, was stimulated with 044747 (5mg/ml) as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.|Lane 1: 0min, Lane 2: 10min, Lane 3: 30min, Lane 4: 1hr, Lane 5: 2hr, Lane 6: 4hr, Lane 7: 8hr, Lane 8: 24hr.
DLL1 Active Protein, Western Blot (WB) – Cat# MBS638035

Testing Data of DLL1:Fc active protein

Adipogenesis inhibition. Macroscopic pictures.
DLL1 Active Protein, Testing Data – Cat# MBS638035

Other Notes

Small volumes of DLL1:Fc active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DLL1:Fc purchase

MBS638035 is an active protein targeting DLL1:Fc. Also known as DLL1, Recombinant, Mouse:Fc (Delta-like Protein 1, Delta 1). Host: Human, Mouse, HEK 293 cells.
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