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Beta-1,4-Galactosyltransferase 1 (B4GALT1) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638056

Beta-1,4-Galactosyltransferase 1 (B4GalT1) is one of seven beta1, 4 galactosyltransferases that transfer galactose in a beta1,4 linkage to acceptor sugars including Gl cNAc, and Gl c, and Xyl. By sequence similarity, the B4GalTs form four groups: B4GalT1 and… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Beta-1,4-Galactosyltransferase 1 (B4GALT1), Active Protein
Full Product Name
Beta-1,4-Galactosyltransferase 1, Recombinant, Human (B4GalT1)
Product Gene Name
B4GALT1 active protein
Form/Format
Supplied as a liquid in 25mM Tris, 150mM sodium chloride, pH 7.5.
Purity/Purification
Highly Purified
95% by SDS­PAGE under reducing conditions and visualized by silver stain.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
40kD
Chromosome Location
Chromosome: 8; AC_000165.1 (76179270..76232552, complement). Location: chromosome: 8
3D Structure
ModBase 3D Structure for P08037
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Immunogen
NS0-derived recombinant human B4GalT1, NP_001488.
Activity
Measured by its ability to transfer galactose from UDP Galactose to N-Acetyl-alpha-D-glucosamine. The specific activity is >2000pmol/min/ug, as measured under the described conditions. See protocol.
Endotoxin Level
1EU/ug (LAL)

Product Categories/Family for B4GALT1 active protein

Molecular Biology; MB-Enzymes
Application Notes for B4GALT1 active protein
SDS-PAGE: 45 55kD

Other Notes

Small volumes of B4GALT1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for B4GALT1 purchase

MBS638056 is an active protein targeting B4GALT1. Also known as Beta-1,4-Galactosyltransferase 1, Recombinant, Human (B4GalT1).

Additional Details

NCBI/Uniprot data below describe general gene information for B4GALT1. It may not necessarily be applicable to this product.

NCBI GI #
29135321
NCBI GeneID
281781
NCBI Accession #
NP_803478.1
UniProt Primary Accession #
P08037
UniProt Secondary Accession #
Q0VC05; Q8MIG0
UniProt Related Accession #
P08037
GenBank Nucleotide #
NM_177512.2
NCBI Official Full Name
beta-1,4-galactosyltransferase 1
NCBI Official Synonym Full Names
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
NCBI Official Symbol
B4GALT1
NCBI Official Synonym Symbols
GGTB2
UniProt Protein Name
Beta-1,4-galactosyltransferase 1
UniProt Synonym Protein Names
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1Cleaved into the following chain:Processed beta-1,4-galactosyltransferase 1Including the following 4 domains:Lactose synthase A protein (EC:2.4.1.22); N-acetyllactosamine synthase (EC:2.4.1.90)Alternative name(s):Nal synthase
Protein Family
Beta-1,4-galactosyltransferase
UniProt Gene Name
B4GALT1
UniProt Synonym Gene Names
GALT; GGTB2; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1
UniProt Entry Name
B4GT1_BOVIN
NCBI Protein Information
beta-1,4-galactosyltransferase 1; b4Gal-T1; beta4Gal-T1; beta-1,4-GalTase 1; beta-galactosyltransferase 2 (glycoprotein); glycoprotein-4-beta-galactosyltransferase 2; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

UniProt Comments for B4GALT1

Function: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

Catalytic activity: UDP-alpha-D-galactose + D-glucose = UDP + lactose.UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactor: Manganese.

Pathway: Protein modification; protein glycosylation.

Subunit structure: Homodimer; and heterodimer with alpha-lactabulmin to form lactose synthase.

Subcellular location: Isoform Long: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Note: Found in trans cisternae of Golgi.Isoform Short: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Found in trans cisternae of Golgi.Processed beta-1,4-galactosyltransferase 1: Secreted. Note: Soluble form found in body fluids.

Post-translational modification: The soluble form derives from the membrane forms by proteolytic processing.

Sequence similarities: Belongs to the glycosyltransferase 7 family.
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Research Articles on B4GALT1

1. the beta4galt1 gene is potentially associated with milk production traits in dairy cows and should be considered for further studies on genetics of the milk production traits.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with B4GALT1 active protein

 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Fertilization Pathway antibodies  Fertilization Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
 Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram

Diseases associated with B4GALT1 active protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with B4GALT1 and Neoplasms
 Neoplasms, Experimental Antibodies  >2 publications with B4GALT1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >2 publications with B4GALT1 and Breast Neoplasms
 Inflammation Antibodies  >2 publications with B4GALT1 and Inflammation
 Disease Models, Animal Antibodies  >1 publications with B4GALT1 and Disease Models, Animal
 Prostatic Neoplasms Antibodies  >1 publications with B4GALT1 and Prostatic Neoplasms
 Infertility, Female Antibodies  >1 publications with B4GALT1 and Infertility, Female
 Neoplasm Metastasis Antibodies  >1 publications with B4GALT1 and Neoplasm Metastasis
 Liver Diseases Antibodies  >1 publications with B4GALT1 and Liver Diseases
 Prostatic Diseases Antibodies  >1 publications with B4GALT1 and Prostatic Diseases

Organs/Tissues associated with B4GALT1 active protein

 Organ/Tissue Name  Pubmed Publications
 Reticulum Antibodies  >3 publications with B4GALT1 and Reticulum
 Liver Antibodies  >2 publications with B4GALT1 and Liver
 Mammary Gland Antibodies  >2 publications with B4GALT1 and Mammary Gland
 Adrenal Gland Antibodies  >1 publications with B4GALT1 and Adrenal Gland
 Lung Antibodies  >1 publications with B4GALT1 and Lung
 Uterus Antibodies  >1 publications with B4GALT1 and Uterus
 Ovary Antibodies  >1 publications with B4GALT1 and Ovary
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