ASAHL Active Protein

The human ASAHL gene encodes N-acylethanolamine-hydrolyzing acid amidase (NAAA), a fatty acid amidase with maximal activity at acidic pH (1). NAAA hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl, N-oleoyl, and N-arachidonoyl, but is most active against N-palmitoylethanolamine (2). NAAA is a member of the cholylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (3). NAAA is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (3). NAAA can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonyl fluorophosphonate (2).

Recombinant human N-acylethanolamine-hydrolyzing acid amidase (NAAA) encoded by the ASAHL gene (rhASAHL; amino acid residues 1-359; Accession # Q02083; variant V151I) was expressed with a C-terminal 10X His tag in a Chinese Hamster Ovary cell line.
Calculated molecular mass of 39kD and migrates as a 50kD band in SDS-PAGE under reducing conditions. Fragments of 33 and 2 kD, resulting from proteolytic processing during maturation, are also present.

Small volumes of ASAHL active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS638135 is an active protein targeting ASAHL. Also known as ASAHL, Recombinant, Human (N-Acylsphingosine Amidohydrolase-like). Host: Recombinant Human.