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Protein Kinase G1 Beta, N-Terminal HIS Tag Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638140

MBS638140 is an active protein targeting Protein Kinase G1 beta, N-terminal HIS tag. Also known as Protein Kinase G1 beta, N-terminal HIS tag, Recombinant, Human (PKG1 beta, Cyclic GMP-dependent Protein Kinase). More details

$1060 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Protein Kinase G1 beta, N-terminal HIS tag, Active Protein
Full Product Name
Protein Kinase G1 beta, N-terminal HIS tag, Recombinant, Human (PKG1 beta, Cyclic GMP-dependent Protein Kinase)
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 0.05% tween 20, 3mM DTT, 20% glycerol.
Purity/Purification
Purified
Purified ~70%
Preparation and Storage
Aliquot to avoid repeated freezing and thawing. Store at -70 degree C. Aliquots are stable for at least 12 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
78kD
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
0.85pmoles/min/ug. Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 2uM substrate (SER/THR 14) at RT for 1hour.

Product Categories/Family for Protein Kinase G1 beta, N-terminal HIS tag active protein

Molecular Biology; MB-Protein Kinases
Application Notes for Protein Kinase G1 beta, N-terminal HIS tag active protein
Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Testing Data of Protein Kinase G1 beta, N-terminal HIS tag active protein

specific activity
Active Protein – Cat# MBS638140

SDS-Page of Protein Kinase G1 beta, N-terminal HIS tag active protein

10% SDS page coomassie staining using P9102-95R. Lane 1: 3ug PKG1 beta. Lane 2: Protein marker.
Active Protein, SDS-Page – Cat# MBS638140

Other Notes

Small volumes of Protein Kinase G1 beta, N-terminal HIS tag active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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