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DEP1 Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638238

Catalytic domain, (a.a. 1024-1306), MW=32.9kD, expressed in an E. coli expression system. More details

$1065 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DEP1, Active Protein
Full Product Name
DEP1 (1024-1306), protein tyrosine phosphatase, catalytic domain, Recombinant, Human (Density-enhanced Phosphatase-1, PTP-eta, CD148)
Product Gene Name
Product Synonym Names
DEP1 (1024-1306), protein tyrosine phosphatase, catalytic domain, Recombinant, Human (Density-enhanced Phosphatase-1, PTP-eta, CD148)
Host
Recombinant, Human from E Coli
Species Reactivity
Human
Form/Format
25 mM Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 3mM DTT.
Purity/Purification
>95% by SDS-PAGE.
Preparation and Storage
-70 degree C
Stability: 6 months at -20 degree C
Molecular Weight
46,978 Da
Chromosome Location
Chromosome: I; NC_001133.9 (129270..130487). Location: chromosome: I
3D Structure
ModBase 3D Structure for P31385
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
114 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 degree C.
Assay Buffer
50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50mM pNPP.
Application Notes for DEP1 active protein
useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

Other Notes

Small volumes of DEP1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEP1 purchase

MBS638238 is an active protein targeting DEP1. Also known as DEP1 (1024-1306), protein tyrosine phosphatase, catalytic domain, Recombinant, Human (Density-enhanced Phosphatase-1, PTP-eta, CD148). Reactive in Human. Host: Recombinant, Human from E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DEP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Dep1p
NCBI Official Symbol
DEP1
NCBI Official Synonym Symbols
FUN54
UniProt Protein Name
Transcriptional regulatory protein DEP1
UniProt Gene Name
DEP1
UniProt Synonym Gene Names
FUN54
UniProt Entry Name
DEP1_YEAST
NCBI Protein Information
Dep1p

UniProt Comments for DEP1

Function: Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Ref.7

Subunit structure: Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6. Ref.8 Ref.9

Subcellular location: Cytoplasm. Nucleus.

Sequence caution: The sequence AAC04944.2 differs from that shown. Reason: Frameshift at position 405.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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