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HIV-1 Env Gp160, Strain YU2 (HIV) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638272

Gene cloned from Macrophage-cell-tropic HIV-1 YU2 strain and the full length (membrane anchor truncation) recombinant envelope gp160 glycoprotein produced in the Baculovirus Expression System. More details

$1300 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIV-1 env gp160, Strain YU2, Active Protein
Full Product Name
HIV-1 env gp160, Strain YU2 (M Tropic), Recombinant (Human Immunodeficiency Virus-1)
Host
Baculovirus
Form/Format
Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography to 95% purity as determined by SDS-PAGE, reduced.
Specificity
This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp160/Anti gp160 mAb-peroxidase capture ELISA. Cell activation Assays pending.
A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.

Other Notes

Small volumes of HIV-1 env gp160, Strain YU2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIV-1 env gp160, Strain YU2 purchase

MBS638272 is an active protein targeting HIV-1 env gp160, Strain YU2. Also known as HIV-1 env gp160, Strain YU2 (M Tropic), Recombinant (Human Immunodeficiency Virus-1). Host: Baculovirus.
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