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HIV-1 Env Gp120, Strain IIIB (HIV) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638277

MBS638277 is an active protein targeting HIV-1 env gp120, Strain IIIB. Also known as HIV-1 env gp120, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin). More details

$1000 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HIV-1 env gp120, Strain IIIB, Active Protein
Full Product Name
HIV-1 env gp120, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)
Form/Format
Supplied as a liquid in PBS,0.2% BSA, pH 7.5, 0.09 sodium azide. Labeled with Biotin.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography to 95% purity as determined by SDS-PAGE, reduced.
Concentration
1 mg/ml (lot specific)
Specificity
Recognized by human and mouse anti-gp120 monoclonal antibodies.
Preparation and Storage
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Coupling Ratio
~5-20 moles of biotin per mole gp 120.
Biological Activity
Biotin gp120 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.

Product Categories/Family for HIV-1 env gp120, Strain IIIB active protein

Molecular Biology; MB-Infectious Disease
Applications Tested/Suitable for HIV-1 env gp120, Strain IIIB active protein
Suitable for use in T-cell Research, ELISA and Western Blot.
Other applications not tested.
Application Notes for HIV-1 env gp120, Strain IIIB active protein
ELISA: 1:10,000
Capture ELISA:10-1000ng,using 100ng of CD4 in solid phase.
Optimal dilutions to be determined by the researcher.

Other Notes

Small volumes of HIV-1 env gp120, Strain IIIB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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