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Eco81i Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638344

MBS638344 is a restriction enzyme targeting Eco81I. Also known as Eco81I (SauI). 5'-C C^T N A G G-3' More details

$470 / 500 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Eco81I, Restriction Enzyme
Full Product Name
Eco81I (SauI)
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Digestion of Agarose-embedded DNA
A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends
Bpu10I, Bpu1102I, DdeI
Blue/White Cloning Assay
The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda
2
Ligation/Recutting Assay
After 10-fold overdigestion (5u/ug DNA x 2 hours) with Eco81I, more than 80% of the M13mp18 DNA fragments can be ligated in a reaction mixture containing 20-40u of T4 DNA Ligase/1ug of fragments and 10% PEG at a 5'-termini concentration of 0.3uM. More than 95% of these can be recut.
M13mp18/19
1
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with Eco81I.
pBR322
0
PhiX174
0
pTZ19R/U
0
pUC18/19
0
pUC57
0
Stability During Prolonged Incubation
A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Enzyme is inactivated by incubation at 80 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-HindIII fragments in 1 hour at 37 degree C in 50ul of assay buffer.

Product Categories/Family for Eco81I restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

Testing Data of Eco81I restriction enzyme

Restriction Enzyme – Cat# MBS638344

Other Notes

Small volumes of Eco81I restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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